7D0K

The icosahedral capsid of Omono River virus (strain:LZ)

Summary for 7D0K

• Entry DOI: 10.2210/pdb7d0k/pdb
• EMDB information: 30537
• Descriptor: Capsid protein (1 entity in total)
• Functional Keywords: totiviridae, major capsid, virus
• Biological source: Omono River virus
• Total number of polymer chains: 2
• Total formula weight: 193721.98

Authors

Shao, Q.,Jia, X.,Gao, Y.,Liu, Z. (deposition date: 2020-09-11, release date: 2021-03-03, Last modification date: 2024-03-27)

Primary citation

Shao, Q.,Jia, X.,Gao, Y.,Liu, Z.,Zhang, H.,Tan, Q.,Zhang, X.,Zhou, H.,Li, Y.,Zhang, Q.
Cryo-EM reveals a previously unrecognized structural protein of a dsRNA virus implicated in its extracellular transmission.
Plos Pathog., 17:e1009396-e1009396, 2021

PubMed Abstract: Mosquito viruses cause unpredictable outbreaks of disease. Recently, several unassigned viruses isolated from mosquitoes, including the Omono River virus (OmRV), were identified as totivirus-like viruses, with features similar to those of the Totiviridae family. Most reported members of this family infect fungi or protozoans and lack an extracellular life cycle stage. Here, we identified a new strain of OmRV and determined high-resolution structures for this virus using single-particle cryo-electron microscopy. The structures feature an unexpected protrusion at the five-fold vertex of the capsid. Disassociation of the protrusion could result in several conformational changes in the major capsid. All these structures, together with some biological results, suggest the protrusions' associations with the extracellular transmission of OmRV.

PubMed: 33730056
DOI: 10.1371/journal.ppat.1009396

Experimental method

ELECTRON MICROSCOPY (2.79 Å)