7CYC

Cryo-EM structures of Alphacoronavirus spike glycoprotein

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7CYC の概要

• エントリーDOI: 10.2210/pdb7cyc/pdb
• EMDBエントリー: 30496
• 分子名称: Spike glycoprotein, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: alphacoronavirus, spike glycoprotein, structural protein
• 由来する生物種: Human coronavirus 229E (HCoV-229E)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 386321.78

構造登録者

Song, X.,Shi, Y.,Ding, W.,Liu, Z.J.,Peng, G. (登録日: 2020-09-03, 公開日: 2020-12-23, 最終更新日: 2024-11-06)

主引用文献

Song, X.,Shi, Y.,Ding, W.,Niu, T.,Sun, L.,Tan, Y.,Chen, Y.,Shi, J.,Xiong, Q.,Huang, X.,Xiao, S.,Zhu, Y.,Cheng, C.,Fu, Z.F.,Liu, Z.J.,Peng, G.
Cryo-EM analysis of the HCoV-229E spike glycoprotein reveals dynamic prefusion conformational changes.
Nat Commun, 12:141-141, 2021

PubMed Abstract: Coronaviruses spike (S) glycoproteins mediate viral entry into host cells by binding to host receptors. However, how the S1 subunit undergoes conformational changes for receptor recognition has not been elucidated in Alphacoronavirus. Here, we report the cryo-EM structures of the HCoV-229E S trimer in prefusion state with two conformations. The activated conformation may pose the potential exposure of the S1-RBDs by decreasing of the interaction area between the S1-RBDs and the surrounding S1-NTDs and S1-RBDs compared to the closed conformation. Furthermore, structural comparison of our structures with the previously reported HCoV-229E S structure showed that the S trimers trended to open the S2 subunit from the closed conformation to open conformation, which could promote the transition from pre- to postfusion. Our results provide insights into the mechanisms involved in S glycoprotein-mediated Alphacoronavirus entry and have implications for vaccine and therapeutic antibody design.

PubMed: 33420048
DOI: 10.1038/s41467-020-20401-y

実験手法

ELECTRON MICROSCOPY (3.21 Å)