7CJ8

Crystal structure of N-terminal His-tagged D-allulose 3-epimerase from Methylomonas sp. in complex with D-allulose

Summary for 7CJ8

• Entry DOI: 10.2210/pdb7cj8/pdb
• Related: 7CJ4
• Descriptor: Epimerase, MANGANESE (II) ION, 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID, ... (5 entities in total)
• Functional Keywords: epimerase, tim barrel, isomerase
• Biological source: Methylomonas sp. DH-1
• Total number of polymer chains: 6
• Total formula weight: 204396.54

Authors

Yoshida, H.,Yoshihara, A.,Kamitori, S. (deposition date: 2020-07-09, release date: 2021-04-21, Last modification date: 2023-11-29)

Primary citation

Yoshida, H.,Yoshihara, A.,Kato, S.,Mochizuki, S.,Akimitsu, K.,Izumori, K.,Kamitori, S.
Crystal structure of a novel homodimeric l-ribulose 3-epimerase from Methylomonus sp.
Febs Open Bio, 11:1621-1637, 2021

PubMed Abstract: d-Allulose has potential as a low-calorie sweetener which can suppress fat accumulation. Several enzymes capable of d-allulose production have been isolated, including d-tagatose 3-epimerases. Here, we report the isolation of a novel protein from Methylomonas sp. expected to be a putative enzyme based on sequence similarity to ketose 3-epimerase. The synthesized gene encoding the deduced ketose 3-epimerase was expressed as a recombinant enzyme in Escherichia coli, and it exhibited the highest enzymatic activity toward l-ribulose, followed by d-ribulose and d-allulose. The X-ray structure analysis of l-ribulose 3-epimerase from Methylomonas sp. (MetLRE) revealed a homodimeric enzyme, the first reported structure of dimeric l-ribulose 3-epimerase. The monomeric structure of MetLRE is similar to that of homotetrameric l-ribulose 3-epimerases, but the short C-terminal α-helix of MetLRE is unique and different from those of known l-ribulose 3 epimerases. The length of the C-terminal α-helix was thought to be involved in tetramerization and increasing stability; however, the addition of residues to MetLRE at the C terminus did not lead to tetramer formation. MetLRE is the first dimeric l-ribulose 3-epimerase identified to exhibit high relative activity toward d-allulose.

PubMed: 33838083
DOI: 10.1002/2211-5463.13159

Experimental method

X-RAY DIFFRACTION (2.05 Å)