7CEH

Crystal structure of PET-degrading cutinase Cut190 S176A/S226P/R228S/ mutant with the C-terminal three residues deletion in ligand ejecting form

Summary for 7CEH

• Entry DOI: 10.2210/pdb7ceh/pdb
• Related: 7CEF
• Descriptor: Alpha/beta hydrolase family protein, CALCIUM ION (3 entities in total)
• Functional Keywords: protein engineering, thermostability, polyesterase, multiple conformation, hydrolase
• Biological source: Saccharomonospora viridis
• Total number of polymer chains: 1
• Total formula weight: 28917.38

Authors

Senga, A.,Numoto, N.,Ito, N.,Kawai, F.,Oda, M. (deposition date: 2020-06-23, release date: 2020-08-26, Last modification date: 2023-11-29)

• Primary citation: Senga, A.,Numoto, N.,Yamashita, M.,Iida, A.,Ito, N.,Kawai, F.,Oda, M.; Multiple structural states of Ca2+-regulated PET hydrolase, Cut190, and its correlation with activity and stability.; J.Biochem., 169:207-213, 2021; PubMed: 32882044; DOI: 10.1093/jb/mvaa102

Experimental method

X-RAY DIFFRACTION (1.09 Å)