7CEH

Crystal structure of PET-degrading cutinase Cut190 S176A/S226P/R228S/ mutant with the C-terminal three residues deletion in ligand ejecting form

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Alpha/beta hydrolase family protein	polymer	262	28837.2	1	UniProt (W0TJ64) Pfam (PF03403) In PDB	Saccharomonospora viridis	Cutinase
2	A	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
3		water	water		18.0	341	Chemie (HOH)

Sequence modifications

A: 45 - 304 (UniProt: W0TJ64)

PDB	External Database	Details
Gly 43	-	expression tag
Pro 44	-	expression tag
Ala 176	Ser 176	engineered mutation
Pro 226	Ser 226	engineered mutation
Ser 228	Arg 228	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	28837.2
Non-Polymers*	Number of molecules	2
	Total formula weight	80.2
All*	Total formula weight	28917.4

*Water molecules are not included.