Loading
PDBj
MenuPDBj@FacebookPDBj@X(formerly Twitter)PDBj@BlueSkyPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

7C06

Crystal structure of yeast U2AF1 complex bound to 3' splice site RNA, 5'-UAGGU.

Summary for 7C06
Entry DOI10.2210/pdb7c06/pdb
DescriptorSplicing factor U2AF 23 kDa subunit, Splicing factor U2AF 59 kDa subunit, RNA (5'-R(*U*UP*AP*GP*GP*U)-3'), ... (4 entities in total)
Functional Keywordspre-mrna, splicing, intron, 3' splice site, zinc-finger, splicing-rna complex, splicing/rna
Biological sourceSchizosaccharomyces pombe 972h- (Fission yeast)
More
Total number of polymer chains27
Total formula weight314271.93
Authors
Yoshida, H.,Park, S.Y.,Urano, T.,Obayashi, E. (deposition date: 2020-04-30, release date: 2020-09-30, Last modification date: 2023-11-29)
Primary citationYoshida, H.,Park, S.Y.,Sakashita, G.,Nariai, Y.,Kuwasako, K.,Muto, Y.,Urano, T.,Obayashi, E.
Elucidation of the aberrant 3' splice site selection by cancer-associated mutations on the U2AF1.
Nat Commun, 11:4744-4744, 2020
Cited by
PubMed Abstract: The accurate exclusion of introns by RNA splicing is critical for the production of mature mRNA. U2AF1 binds specifically to the 3´ splice site, which includes an essential AG dinucleotide. Even a single amino acid mutation of U2AF1 can cause serious disease such as certain cancers or myelodysplastic syndromes. Here, we describe the first crystal structures of wild-type and pathogenic mutant U2AF1 complexed with target RNA, revealing the mechanism of 3´ splice site selection, and how aberrant splicing results from clinically important mutations. Unexpected features of this mechanism may assist the future development of new treatments against diseases caused by splicing errors.
PubMed: 32958768
DOI: 10.1038/s41467-020-18559-6
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (3.02 Å)
Structure validation

227561

건을2024-11-20부터공개중

PDB statisticsPDBj update infoContact PDBjnumon