7C06
Crystal structure of yeast U2AF1 complex bound to 3' splice site RNA, 5'-UAGGU.
Summary for 7C06
Entry DOI | 10.2210/pdb7c06/pdb |
Descriptor | Splicing factor U2AF 23 kDa subunit, Splicing factor U2AF 59 kDa subunit, RNA (5'-R(*U*UP*AP*GP*GP*U)-3'), ... (4 entities in total) |
Functional Keywords | pre-mrna, splicing, intron, 3' splice site, zinc-finger, splicing-rna complex, splicing/rna |
Biological source | Schizosaccharomyces pombe 972h- (Fission yeast) More |
Total number of polymer chains | 27 |
Total formula weight | 314271.93 |
Authors | Yoshida, H.,Park, S.Y.,Urano, T.,Obayashi, E. (deposition date: 2020-04-30, release date: 2020-09-30, Last modification date: 2023-11-29) |
Primary citation | Yoshida, H.,Park, S.Y.,Sakashita, G.,Nariai, Y.,Kuwasako, K.,Muto, Y.,Urano, T.,Obayashi, E. Elucidation of the aberrant 3' splice site selection by cancer-associated mutations on the U2AF1. Nat Commun, 11:4744-4744, 2020 Cited by PubMed Abstract: The accurate exclusion of introns by RNA splicing is critical for the production of mature mRNA. U2AF1 binds specifically to the 3´ splice site, which includes an essential AG dinucleotide. Even a single amino acid mutation of U2AF1 can cause serious disease such as certain cancers or myelodysplastic syndromes. Here, we describe the first crystal structures of wild-type and pathogenic mutant U2AF1 complexed with target RNA, revealing the mechanism of 3´ splice site selection, and how aberrant splicing results from clinically important mutations. Unexpected features of this mechanism may assist the future development of new treatments against diseases caused by splicing errors. PubMed: 32958768DOI: 10.1038/s41467-020-18559-6 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (3.02 Å) |
Structure validation
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