7BX2
The solution NMR structure of VV14 peptide in the presence of Deuterated SDS micelle.
Summary for 7BX2
| Entry DOI | 10.2210/pdb7bx2/pdb |
| NMR Information | BMRB: 36347 |
| Descriptor | VAL-LYS-TRP-VAL-LYS-LYS-VAL-VAL-LYS-TRP-VAL-LYS-LYS-VAL (1 entity in total) |
| Functional Keywords | structure from cyana 2.1, antimicrobial peptide, sds micelle, alpha helix., antimicrobial protein |
| Biological source | synthetic construct |
| Total number of polymer chains | 1 |
| Total formula weight | 1760.30 |
| Authors | Bhunia, A.,Mohid, S.A.,Chowdhury, N. (deposition date: 2020-04-16, release date: 2021-04-21, Last modification date: 2024-05-15) |
| Primary citation | Pandit, G.,Chowdhury, N.,Abdul Mohid, S.,Bidkar, A.P.,Bhunia, A.,Chatterjee, S. Effect of Secondary Structure and Side Chain Length of Hydrophobic Amino Acid Residues on the Antimicrobial Activity and Toxicity of 14-Residue-Long de novo AMPs. Chemmedchem, 16:355-367, 2021 Cited by PubMed Abstract: Herein we report the efficacy and toxicity of three de novo designed cationic antimicrobial peptides (AMPs) LL-14, VV-14 and ββ-14, where side chains of the hydrophobic amino acids were reduced gradually. The AMPs showed broad-spectrum antimicrobial activity against three pathogens from the ESKAPE group and two fungal strains. This study showed that side chains which are either too long or too short increase toxicity and lower antimicrobial activity, respectively. VV-14 was found to be non-cytotoxic and highly potent under physiological salt concentrations against several pathogens, especially Salmonella typhi TY2. These AMPs acted via membrane deformation, depolarization, and lysis. The activity of the AMPs is related to their ability to take on amphipathic helical conformations in the presence of microbial membrane mimics. Among AMPs with the same charge, hydrophobic interactions between the side chains of the residues with cell membrane lipids determine their antimicrobial potency and cytotoxicity. Strikingly, an optimum hydrophobic interaction is the crux of generating highly potent non-cytotoxic AMPs. PubMed: 33026188DOI: 10.1002/cmdc.202000550 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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