7BWN

Crystal Structure of a Designed Protein Heterocatenane

7BWN の概要

• エントリーDOI: 10.2210/pdb7bwn/pdb
• 分子名称: Chimera of Green fluorescent protein and p53dim, Cellular tumor antigen p53 (3 entities in total)
• 機能のキーワード: heterocatenane, recombination
• 由来する生物種: Aequorea victoria (Jellyfish); 詳細
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 297092.54

構造登録者

Liu, Y.J.,Duan, Z.L.,Fang, J.,Zhang, F.,Xiao, J.Y.,Zhang, W.B. (登録日: 2020-04-15, 公開日: 2020-06-17, 最終更新日: 2023-11-29)

主引用文献

Liu, Y.,Duan, Z.,Fang, J.,Zhang, F.,Xiao, J.,Zhang, W.B.
Cellular Synthesis and X-ray Crystal Structure of a Designed Protein Heterocatenane.
Angew.Chem.Int.Ed.Engl., 59:16122-16127, 2020

PubMed Abstract: Herein, we report the biosynthesis of protein heterocatenanes using a programmed sequence of multiple post-translational processing events including intramolecular chain entanglement, in situ backbone cleavage, and spontaneous cyclization. The approach is general, autonomous, and can obviate the need for any additional enzymes. The catenane topology was convincingly proven using a combination of SDS-PAGE, LC-MS, size exclusion chromatography, controlled proteolytic digestion, and protein crystallography. The X-ray crystal structure clearly shows two mechanically interlocked protein rings with intact folded domains. It opens new avenues in the nascent field of protein-topology engineering.

PubMed: 32506656
DOI: 10.1002/anie.202005490

実験手法

X-RAY DIFFRACTION (2.396 Å)