7BTB

Cryo-EM structure of pre-60S ribosome from Saccharomyces cerevisiae rpl4delta63-87 strain at 3.22 Angstroms resolution(state R2)

This is a non-PDB format compatible entry.

Summary for 7BTB

• Entry DOI: 10.2210/pdb7btb/pdb
• EMDB information: 30174
• Descriptor: 60S ribosomal protein L13-A, 60S ribosomal protein L9-A, 60S ribosomal protein L11-A, ... (56 entities in total)
• Functional Keywords: pre-60s, rpl4, ribosome
• Biological source: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast); More
• Total number of polymer chains: 53
• Total formula weight: 2517110.28

Authors

Li, Y.,Wilson, D.M. (deposition date: 2020-04-01, release date: 2020-10-28, Last modification date: 2024-10-16)

Primary citation

Wilson, D.M.,Li, Y.,LaPeruta, A.,Gamalinda, M.,Gao, N.,Woolford Jr., J.L.
Structural insights into assembly of the ribosomal nascent polypeptide exit tunnel.
Nat Commun, 11:5111-5111, 2020

PubMed Abstract: The nascent polypeptide exit tunnel (NPET) is a major functional center of 60S ribosomal subunits. However, little is known about how the NPET is constructed during ribosome assembly. We utilized molecular genetics, biochemistry, and cryo-electron microscopy (cryo-EM) to investigate the functions of two NPET-associated proteins, ribosomal protein uL4 and assembly factor Nog1, in NPET assembly. Structures of mutant pre-ribosomes lacking the tunnel domain of uL4 reveal a misassembled NPET, including an aberrantly flexible ribosomal RNA helix 74, resulting in at least three different blocks in 60S assembly. Structures of pre-ribosomes lacking the C-terminal extension of Nog1 demonstrate that this extension scaffolds the tunnel domain of uL4 in the NPET to help maintain stability in the core of pre-60S subunits. Our data reveal that uL4 and Nog1 work together in the maturation of ribosomal RNA helix 74, which is required to ensure proper construction of the NPET and 60S ribosomal subunits.

PubMed: 33037216
DOI: 10.1038/s41467-020-18878-8

Experimental method

ELECTRON MICROSCOPY (3.22 Å)