7BHO

DNA origami signpost designed model

This is a non-PDB format compatible entry.

Summary for 7BHO

• Entry DOI: 10.2210/pdb7bho/pdb
• EMDB information: 12188
• Descriptor: DNA, DNA scaffold, ... (238 entities in total)
• Functional Keywords: origami, nanostructure, signpost, spot, dna
• Biological source: Escherichia virus M13; More
• Total number of polymer chains: 238
• Total formula weight: 4453172.71

Authors

Silvester, E.,Vollmer, B.,Prazak, V.,Vasishtan, D.,Machala, E.A.,Whittle, C.,Black, S.,Bath, J.,Turberfield, A.J.,Gruenewald, K.,Baker, L.A. (deposition date: 2021-01-11, release date: 2021-04-14, Last modification date: 2024-05-01)

Primary citation

Silvester, E.,Vollmer, B.,Prazak, V.,Vasishtan, D.,Machala, E.A.,Whittle, C.,Black, S.,Bath, J.,Turberfield, A.J.,Grunewald, K.,Baker, L.A.
DNA origami signposts for identifying proteins on cell membranes by electron cryotomography.
Cell, 184:1110-1121.e16, 2021

PubMed Abstract: Electron cryotomography (cryoET), an electron cryomicroscopy (cryoEM) modality, has changed our understanding of biological function by revealing the native molecular details of membranes, viruses, and cells. However, identification of individual molecules within tomograms from cryoET is challenging because of sample crowding and low signal-to-noise ratios. Here, we present a tagging strategy for cryoET that precisely identifies individual protein complexes in tomograms without relying on metal clusters. Our method makes use of DNA origami to produce "molecular signposts" that target molecules of interest, here via fluorescent fusion proteins, providing a platform generally applicable to biological surfaces. We demonstrate the specificity of signpost origami tags (SPOTs) in vitro as well as their suitability for cryoET of membrane vesicles, enveloped viruses, and the exterior of intact mammalian cells.

PubMed: 33606980
DOI: 10.1016/j.cell.2021.01.033

Experimental method

ELECTRON MICROSCOPY (36.44 Å)