7BH7

Room temperature, serial X-ray structure of the ertapenem-derived acylenzyme of CTX-M-15 (10 min soak) collected on fixed target chips at Diamond Light Source I24

Summary for 7BH7

• Entry DOI: 10.2210/pdb7bh7/pdb
• Descriptor: Beta-lactamase, (2~{S},3~{R},4~{R})-3-[5-[(3-carboxyphenyl)carbamoyl]pyrrolidin-3-yl]sulfanyl-4-methyl-5-[(2~{S},3~{R})-3-oxidanyl-1-oxidanylidene-butan-2-yl]-3,4-dihydro-2~{H}-pyrrole-2-carboxylic acid, CHLORIDE ION, ... (5 entities in total)
• Functional Keywords: serial crystallography, beta-lactamase, antibiotic, ertapenem, antimicrobial protein
• Biological source: Klebsiella pneumoniae
• Total number of polymer chains: 1
• Total formula weight: 28828.96

Authors

Hinchliffe, P.,Tooke, C.L.,Butryn, A.,Spencer, J. (deposition date: 2021-01-10, release date: 2021-07-21, Last modification date: 2024-01-31)

• Primary citation: Butryn, A.,Simon, P.S.,Aller, P.,Hinchliffe, P.,Massad, R.N.,Leen, G.,Tooke, C.L.,Bogacz, I.,Kim, I.S.,Bhowmick, A.,Brewster, A.S.,Devenish, N.E.,Brem, J.,Kamps, J.J.A.G.,Lang, P.A.,Rabe, P.,Axford, D.,Beale, J.H.,Davy, B.,Ebrahim, A.,Orlans, J.,Storm, S.L.S.,Zhou, T.,Owada, S.,Tanaka, R.,Tono, K.,Evans, G.,Owen, R.L.,Houle, F.A.,Sauter, N.K.,Schofield, C.J.,Spencer, J.,Yachandra, V.K.,Yano, J.,Kern, J.F.,Orville, A.M.; An on-demand, drop-on-drop method for studying enzyme catalysis by serial crystallography.; Nat Commun, 12:4461-4461, 2021; PubMed: 34294694; DOI: 10.1038/s41467-021-24757-7

Experimental method

X-RAY DIFFRACTION (1.65 Å)