7BGJ
C. thermophilum Pyruvate Dehydrogenase Complex Core
Summary for 7BGJ
| Entry DOI | 10.2210/pdb7bgj/pdb |
| EMDB information | 12181 |
| Descriptor | Acetyltransferase component of pyruvate dehydrogenase complex (1 entity in total) |
| Functional Keywords | dihydrolipoyl, transacetylase, e2, pyruvate, transferase |
| Biological source | Chaetomium thermophilum var. thermophilum DSM 1495 |
| Total number of polymer chains | 1 |
| Total formula weight | 48777.49 |
| Authors | Tueting, C.,Kastritis, P.L. (deposition date: 2021-01-07, release date: 2021-02-10, Last modification date: 2024-05-01) |
| Primary citation | Kyrilis, F.L.,Semchonok, D.A.,Skalidis, I.,Tuting, C.,Hamdi, F.,O'Reilly, F.J.,Rappsilber, J.,Kastritis, P.L. Integrative structure of a 10-megadalton eukaryotic pyruvate dehydrogenase complex from native cell extracts. Cell Rep, 34:108727-108727, 2021 Cited by PubMed Abstract: The pyruvate dehydrogenase complex (PDHc) is a giant enzymatic assembly involved in pyruvate oxidation. PDHc components have been characterized in isolation, but the complex's quaternary structure has remained elusive due to sheer size, heterogeneity, and plasticity. Here, we identify fully assembled Chaetomium thermophilum α-keto acid dehydrogenase complexes in native cell extracts and characterize their domain arrangements utilizing mass spectrometry, activity assays, crosslinking, electron microscopy (EM), and computational modeling. We report the cryo-EM structure of the PDHc core and observe unique features of the previously unknown native state. The asymmetric reconstruction of the 10-MDa PDHc resolves spatial proximity of its components, agrees with stoichiometric data (60 E2p:12 E3BP:∼20 E1p: ≤ 12 E3), and proposes a minimum reaction path among component enzymes. The PDHc shows the presence of a dynamic pyruvate oxidation compartment, organized by core and peripheral protein species. Our data provide a framework for further understanding PDHc and α-keto acid dehydrogenase complex structure and function. PubMed: 33567276DOI: 10.1016/j.celrep.2021.108727 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (6.9 Å) |
Structure validation
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