7BFM

Structure of the M198F M298F double mutant of the Streptomyces coelicolor small laccase T1 copper site

Summary for 7BFM

• Entry DOI: 10.2210/pdb7bfm/pdb
• Related: 7B2K 7B4Y
• Descriptor: Putative copper oxidase, TETRAETHYLENE GLYCOL, COPPER (II) ION, ... (4 entities in total)
• Functional Keywords: small laccase, oxidoreductase, t1 copper, double mutant, m198f m298f
• Biological source: Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
• Total number of polymer chains: 2
• Total formula weight: 74824.75

Authors

Zovo, K.,Majumdar, S.,Lukk, T. (deposition date: 2021-01-04, release date: 2022-01-12, Last modification date: 2024-01-31)

• Primary citation: Zovo, K.,Pupart, H.,Van Wieren, A.,Gillilan, R.E.,Huang, Q.,Majumdar, S.,Lukk, T.; Substitution of the Methionine Axial Ligand of the T1 Copper for the Fungal-like Phenylalanine Ligand (M298F) Causes Local Structural Perturbations that Lead to Thermal Instability and Reduced Catalytic Efficiency of the Small Laccase from Streptomyces coelicolor A3(2).; Acs Omega, 7:6184-6194, 2022; PubMed: 35224382; DOI: 10.1021/acsomega.1c06668

Experimental method

X-RAY DIFFRACTION (2 Å)