7BEF

Structures of class II bacterial transcription complexes

Summary for 7BEF

• Entry DOI: 10.2210/pdb7bef/pdb
• EMDB information: 12156
• Descriptor: DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... (8 entities in total)
• Functional Keywords: antibiotic resistance, bacterial transcription activator, cryoem, transcription
• Biological source: Escherichia coli (strain K12); More
• Total number of polymer chains: 9
• Total formula weight: 522474.51

Authors

Hao, M.,Ye, F.Z.,Zhang, X.D. (deposition date: 2020-12-23, release date: 2021-12-01, Last modification date: 2024-07-10)

Primary citation

Hao, M.,Ye, F.,Jovanovic, M.,Kotta-Loizou, I.,Xu, Q.,Qin, X.,Buck, M.,Zhang, X.,Wang, M.
Structures of Class I and Class II Transcription Complexes Reveal the Molecular Basis of RamA-Dependent Transcription Activation.
Adv Sci, 9:e2103669-e2103669, 2022

PubMed Abstract: Transcription activator RamA is linked to multidrug resistance of Klebsiella pneumoniae through controlling genes that encode efflux pumps (acrA) and porin-regulating antisense RNA (micF). In bacteria, σ , together with activators, controls the majority of genes by recruiting RNA polymerase (RNAP) to the promoter regions. RNAP and σ form a holoenzyme that recognizes -35 and -10 promoter DNA consensus sites. Many activators bind upstream from the holoenzyme and can be broadly divided into two classes. RamA acts as a class I activator on acrA and class II activator on micF, respectively. The authors present biochemical and structural data on RamA in complex with RNAP-σ at the two promoters and the data reveal the molecular basis for how RamA assembles and interacts with core RNAP and activates transcription that contributes to antibiotic resistance. Further, comparing with CAP/TAP complexes reveals common and activator-specific features in activator binding and uncovers distinct roles of the two C-terminal domains of RNAP α subunit.

PubMed: 34761556
DOI: 10.1002/advs.202103669

Experimental method

ELECTRON MICROSCOPY (4.5 Å)