7B7U

Cryo-EM structure of mammalian RNA polymerase II in complex with human RPAP2

Summary for 7B7U

• Entry DOI: 10.2210/pdb7b7u/pdb
• EMDB information: 12087
• Descriptor: DNA-directed RNA polymerase subunit, RNA polymerase II subunit K, Putative RNA polymerase II subunit B1 CTD phosphatase RPAP2, ... (13 entities in total)
• Functional Keywords: rpap2, rna polymerase ii, nuclear import, transcription
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 12
• Total formula weight: 553875.91

Authors

Fianu, I.,Dienemann, C.,Aibara, S.,Schilbach, S.,Cramer, P. (deposition date: 2020-12-11, release date: 2021-05-19, Last modification date: 2024-11-20)

Primary citation

Fianu, I.,Dienemann, C.,Aibara, S.,Schilbach, S.,Cramer, P.
Cryo-EM structure of mammalian RNA polymerase II in complex with human RPAP2.
Commun Biol, 4:606-606, 2021

PubMed Abstract: Nuclear import of RNA polymerase II (Pol II) involves the conserved factor RPAP2. Here we report the cryo-electron microscopy (cryo-EM) structure of mammalian Pol II in complex with human RPAP2 at 2.8 Å resolution. The structure shows that RPAP2 binds between the jaw domains of the polymerase subunits RPB1 and RPB5. RPAP2 is incompatible with binding of downstream DNA during transcription and is displaced upon formation of a transcription pre-initiation complex.

PubMed: 34021257
DOI: 10.1038/s42003-021-02088-z

Experimental method

ELECTRON MICROSCOPY (2.8 Å)