7B0Y

Structure of a transcribing RNA polymerase II-U1 snRNP complex

7B0Y の概要

• エントリーDOI: 10.2210/pdb7b0y/pdb
• 関連するPDBエントリー: 7B0Y
• EMDBエントリー: 11972
• 分子名称: DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerases I, II, and III subunit RPABC5, DNA-directed RNA polymerase II subunit RPB11-a, ... (27 entities in total)
• 機能のキーワード: transcription, splicing, co-transcriptional spliceosome assembly
• 由来する生物種: Sus scrofa domesticus; 詳細
• タンパク質・核酸の鎖数: 25
• 化学式量合計: 823570.47

構造登録者

Zhang, S.,Aibara, S.,Vos, S.M.,Agafonov, D.E.,Luehrmann, R.,Cramer, P. (登録日: 2020-11-23, 公開日: 2021-01-13, 最終更新日: 2024-05-01)

主引用文献

Zhang, S.,Aibara, S.,Vos, S.M.,Agafonov, D.E.,Luhrmann, R.,Cramer, P.
Structure of a transcribing RNA polymerase II-U1 snRNP complex.
Science, 371:305-309, 2021

PubMed Abstract: To initiate cotranscriptional splicing, RNA polymerase II (Pol II) recruits the U1 small nuclear ribonucleoprotein particle (U1 snRNP) to nascent precursor messenger RNA (pre-mRNA). Here, we report the cryo-electron microscopy structure of a mammalian transcribing Pol II-U1 snRNP complex. The structure reveals that Pol II and U1 snRNP interact directly. This interaction positions the pre-mRNA 5' splice site near the RNA exit site of Pol II. Extension of pre-mRNA retains the 5' splice site, leading to the formation of a "growing intron loop." Loop formation may facilitate scanning of nascent pre-mRNA for the 3' splice site, functional pairing of distant intron ends, and prespliceosome assembly. Our results provide a starting point for a mechanistic analysis of cotranscriptional spliceosome assembly and the biogenesis of mRNA isoforms by alternative splicing.

PubMed: 33446560
DOI: 10.1126/science.abf1870

実験手法

ELECTRON MICROSCOPY (3.6 Å)