7AY8
NMR solution structure of Tbo-IT2
Summary for 7AY8
| Entry DOI | 10.2210/pdb7ay8/pdb |
| NMR Information | BMRB: 34574 |
| Descriptor | Tbo-IT2 (1 entity in total) |
| Functional Keywords | protein, toxin |
| Biological source | Tibellus oblongus |
| Total number of polymer chains | 1 |
| Total formula weight | 4220.97 |
| Authors | Mineev, K.S.,Kornilov, F.D.,Lushpa, V.A.,Korolkova, Y.A.,Maleeva, E.E.,Andreev, Y.A.,Kozlov, S.A.,Arseniev, A.S. (deposition date: 2020-11-11, release date: 2021-03-03, Last modification date: 2024-11-20) |
| Primary citation | Korolkova, Y.,Maleeva, E.,Mikov, A.,Lobas, A.,Solovyeva, E.,Gorshkov, M.,Andreev, Y.,Peigneur, S.,Tytgat, J.,Kornilov, F.,Lushpa, V.,Mineev, K.,Kozlov, S. New Insectotoxin from Tibellus Oblongus Spider Venom Presents Novel Adaptation of ICK Fold. Toxins, 13:-, 2021 Cited by PubMed Abstract: The spider is an active predator that does not spin webs and remains poorly investigated in terms of venom composition. Here, we present a new toxin, named Tbo-IT2, predicted by cDNA analysis of venom glands transcriptome. The presence of Tbo-IT2 in the venom was confirmed by proteomic analyses using the LC-MS and MS/MS techniques. The distinctive features of Tbo-IT2 are the low similarity of primary structure with known animal toxins and the unusual motif of 10 cysteine residues distribution. Recombinant Tbo-IT2 (rTbo-IT2), produced in using the thioredoxin fusion protein strategy, was structurally and functionally studied. rTbo-IT2 showed insecticidal activity on larvae of the housefly (LD 200 μg/g) and no activity on the panel of expressed neuronal receptors and ion channels. The spatial structure of the peptide was determined in a water solution by NMR spectroscopy. The Tbo-IT2 structure is a new example of evolutionary adaptation of a well-known inhibitor cystine knot (ICK) fold to 5 disulfide bonds configuration, which determines additional conformational stability and gives opportunities for insectotoxicity and probably some other interesting features. PubMed: 33406803DOI: 10.3390/toxins13010029 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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