7AU7

Crystal structure of Nod Factor Perception ectodomain

7AU7 の概要

• エントリーDOI: 10.2210/pdb7au7/pdb
• 関連するPDBエントリー: 7BAX
• 分子名称: Serine/threonine receptor-like kinase NFP, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• 機能のキーワード: lysm, plant protein
• 由来する生物種: Medicago truncatula (Barrel medic)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 31885.62

構造登録者

Gysel, K.,Blaise, M.,Andersen, K.R. (登録日: 2020-11-02, 公開日: 2021-11-10, 最終更新日: 2024-11-13)

主引用文献

Gysel, K.,Laursen, M.,Thygesen, M.B.,Lironi, D.,Bozsoki, Z.,Hjuler, C.T.,Maolanon, N.N.,Cheng, J.,Bjork, P.K.,Vinther, M.,Madsen, L.H.,Rubsam, H.,Muszynski, A.,Ghodrati, A.,Azadi, P.,Sullivan, J.T.,Ronson, C.W.,Jensen, K.J.,Blaise, M.,Radutoiu, S.,Stougaard, J.,Andersen, K.R.
Kinetic proofreading of lipochitooligosaccharides determines signal activation of symbiotic plant receptors.
Proc.Natl.Acad.Sci.USA, 118:-, 2021

PubMed Abstract: Plants and animals use cell surface receptors to sense and interpret environmental signals. In legume symbiosis with nitrogen-fixing bacteria, the specific recognition of bacterial lipochitooligosaccharide (LCO) signals by single-pass transmembrane receptor kinases determines compatibility. Here, we determine the structural basis for LCO perception from the crystal structures of two lysin motif receptor ectodomains and identify a hydrophobic patch in the binding site essential for LCO recognition and symbiotic function. We show that the receptor monitors the composition of the amphiphilic LCO molecules and uses kinetic proofreading to control receptor activation and signaling specificity. We demonstrate engineering of the LCO binding site to fine-tune ligand selectivity and correct binding kinetics required for activation of symbiotic signaling in plants. Finally, the hydrophobic patch is found to be a conserved structural signature in this class of LCO receptors across legumes that can be used for in silico predictions. Our results provide insights into the mechanism of cell-surface receptor activation by kinetic proofreading of ligands and highlight the potential in receptor engineering to capture benefits in plant-microbe interactions.

PubMed: 34716271
DOI: 10.1073/pnas.2111031118

実験手法

X-RAY DIFFRACTION (2.547 Å)