7AT8
Histone H3 recognition by nucleosome-bound PRC2 subunit EZH2.
Summary for 7AT8
| Entry DOI | 10.2210/pdb7at8/pdb |
| EMDB information | 11910 |
| Descriptor | Isoform 2 of Histone-lysine N-methyltransferase EZH2,Isoform 2 of Histone-lysine N-methyltransferase EZH2,Histone-lysine N-methyltransferase EZH2,Isoform 2 of Histone-lysine N-methyltransferase EZH2,Isoform 2 of Histone-lysine N-methyltransferase EZH2, S-ADENOSYL-L-HOMOCYSTEINE, Polycomb protein SUZ12, ... (10 entities in total) |
| Functional Keywords | polycomb, nucleosome, histone methyltransferase, prc2, ezh2, h3k36, h3 tail, gene regulation, h3, histone h3, polycomb repressive complex 2, cryo-em, nucleosome recognition, h3k36me2, h3k36me3 |
| Biological source | Homo sapiens (Human) More |
| Total number of polymer chains | 12 |
| Total formula weight | 375667.22 |
| Authors | Finogenova, K.,Benda, C.,Schaefer, I.B.,Poepsel, S.,Strauss, M.,Mueller, J. (deposition date: 2020-10-29, release date: 2020-12-09, Last modification date: 2024-05-01) |
| Primary citation | Finogenova, K.,Bonnet, J.,Poepsel, S.,Schafer, I.B.,Finkl, K.,Schmid, K.,Litz, C.,Strauss, M.,Benda, C.,Muller, J. Structural basis for PRC2 decoding of active histone methylation marks H3K36me2/3. Elife, 9:-, 2020 Cited by PubMed Abstract: Repression of genes by Polycomb requires that PRC2 modifies their chromatin by trimethylating lysine 27 on histone H3 (H3K27me3). At transcriptionally active genes, di- and tri-methylated H3K36 inhibit PRC2. Here, the cryo-EM structure of PRC2 on dinucleosomes reveals how binding of its catalytic subunit EZH2 to nucleosomal DNA orients the H3 N-terminus via an extended network of interactions to place H3K27 into the active site. Unmodified H3K36 occupies a critical position in the EZH2-DNA interface. Mutation of H3K36 to arginine or alanine inhibits H3K27 methylation by PRC2 on nucleosomes . Accordingly, H3K36A and H3K36R mutants show reduced levels of H3K27me3 and defective Polycomb repression of HOX genes. The relay of interactions between EZH2, the nucleosomal DNA and the H3 N-terminus therefore creates the geometry that permits allosteric inhibition of PRC2 by methylated H3K36 in transcriptionally active chromatin. PubMed: 33211010DOI: 10.7554/eLife.61964 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (4.4 Å) |
Structure validation
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