7AT8

Histone H3 recognition by nucleosome-bound PRC2 subunit EZH2.

7AT8 の概要

• エントリーDOI: 10.2210/pdb7at8/pdb
• EMDBエントリー: 11910
• 分子名称: Isoform 2 of Histone-lysine N-methyltransferase EZH2,Isoform 2 of Histone-lysine N-methyltransferase EZH2,Histone-lysine N-methyltransferase EZH2,Isoform 2 of Histone-lysine N-methyltransferase EZH2,Isoform 2 of Histone-lysine N-methyltransferase EZH2, S-ADENOSYL-L-HOMOCYSTEINE, Polycomb protein SUZ12, ... (10 entities in total)
• 機能のキーワード: polycomb, nucleosome, histone methyltransferase, prc2, ezh2, h3k36, h3 tail, gene regulation, h3, histone h3, polycomb repressive complex 2, cryo-em, nucleosome recognition, h3k36me2, h3k36me3
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 375667.22

構造登録者

Finogenova, K.,Benda, C.,Schaefer, I.B.,Poepsel, S.,Strauss, M.,Mueller, J. (登録日: 2020-10-29, 公開日: 2020-12-09, 最終更新日: 2025-12-10)

主引用文献

Finogenova, K.,Bonnet, J.,Poepsel, S.,Schafer, I.B.,Finkl, K.,Schmid, K.,Litz, C.,Strauss, M.,Benda, C.,Muller, J.
Structural basis for PRC2 decoding of active histone methylation marks H3K36me2/3.
Elife, 9:-, 2020

PubMed Abstract: Repression of genes by Polycomb requires that PRC2 modifies their chromatin by trimethylating lysine 27 on histone H3 (H3K27me3). At transcriptionally active genes, di- and tri-methylated H3K36 inhibit PRC2. Here, the cryo-EM structure of PRC2 on dinucleosomes reveals how binding of its catalytic subunit EZH2 to nucleosomal DNA orients the H3 N-terminus via an extended network of interactions to place H3K27 into the active site. Unmodified H3K36 occupies a critical position in the EZH2-DNA interface. Mutation of H3K36 to arginine or alanine inhibits H3K27 methylation by PRC2 on nucleosomes . Accordingly, H3K36A and H3K36R mutants show reduced levels of H3K27me3 and defective Polycomb repression of HOX genes. The relay of interactions between EZH2, the nucleosomal DNA and the H3 N-terminus therefore creates the geometry that permits allosteric inhibition of PRC2 by methylated H3K36 in transcriptionally active chromatin.

PubMed: 33211010
DOI: 10.7554/eLife.61964

実験手法

ELECTRON MICROSCOPY (4.4 Å)