7AR7

Cryo-EM structure of Arabidopsis thaliana complex-I (open conformation)

7AR7 の概要

• エントリーDOI: 10.2210/pdb7ar7/pdb
• EMDBエントリー: 11875
• 分子名称: NADH-ubiquinone oxidoreductase chain 3, NADH-ubiquinone oxidoreductase chain 6, NADH-ubiquinone oxidoreductase chain 4L, ... (59 entities in total)
• 機能のキーワード: complex-i arabidopsis, electron transport
• 由来する生物種: Arabidopsis thaliana (thale cress); 詳細
• タンパク質・核酸の鎖数: 46
• 化学式量合計: 879159.33

構造登録者

Klusch, N.,Kuelbrandt, W. (登録日: 2020-10-23, 公開日: 2022-03-02)

主引用文献

Klusch, N.,Senkler, J.,Yildiz, O.,Kuehlbrandt, W.,Braun, H.P.
A ferredoxin bridge connects the two arms of plant mitochondrial complex I.
Plant Cell, 33:2072-2091, 2021

PubMed Abstract: Mitochondrial complex I is the main site for electron transfer to the respiratory chain and generates much of the proton gradient across the inner mitochondrial membrane. Complex I is composed of two arms, which form a conserved L-shape. We report the structures of the intact, 47-subunit mitochondrial complex I from Arabidopsis thaliana and the 51-subunit complex I from the green alga Polytomella sp., both at around 2.9 Å resolution. In both complexes, a heterotrimeric γ-carbonic anhydrase domain is attached to the membrane arm on the matrix side. Two states are resolved in A. thaliana complex I, with different angles between the two arms and different conformations of the ND1 (NADH dehydrogenase subunit 1) loop near the quinol binding site. The angle appears to depend on a bridge domain, which links the peripheral arm to the membrane arm and includes an unusual ferredoxin. We propose that the bridge domain participates in regulating the activity of plant complex I.

PubMed: 33768254
DOI: 10.1093/plcell/koab092

実験手法

ELECTRON MICROSCOPY (3.72 Å)