7AGR

Structure of the M624V-S726F mutant of AcylTransferase domain of Mycocerosic Acid Synthase from Mycobacterium tuberculosis soaked with MethylMalonyl Coenzyme A

Summary for 7AGR

• Entry DOI: 10.2210/pdb7agr/pdb
• Related: 7AGP 7AGQ 7AGS 7AGT 7AGU
• Descriptor: Mycocerosic acid synthase, 1,2-ETHANEDIOL (3 entities in total)
• Functional Keywords: mycocerosic acid synthase, acyltransferase domain, polyketide synthase, mycobacterium tuberculosis, transferase
• Biological source: Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
• Total number of polymer chains: 2
• Total formula weight: 93058.97

Authors

Brison, Y.,Mourey, L.,Maveyraud, L. (deposition date: 2020-09-23, release date: 2020-12-16, Last modification date: 2024-01-31)

Primary citation

Grabowska, A.D.,Brison, Y.,Maveyraud, L.,Gavalda, S.,Faille, A.,Nahoum, V.,Bon, C.,Guilhot, C.,Pedelacq, J.D.,Chalut, C.,Mourey, L.
Molecular Basis for Extender Unit Specificity of Mycobacterial Polyketide Synthases.
Acs Chem.Biol., 15:3206-3216, 2020

PubMed Abstract: is the causative agent of the tuberculosis disease, which claims more human lives each year than any other bacterial pathogen. and other mycobacterial pathogens have developed a range of unique features that enhance their virulence and promote their survival in the human host. Among these features lies the particular cell envelope with high lipid content, which plays a substantial role in mycobacterial pathogenicity. Several envelope components of and other mycobacteria, e.g., mycolic acids, phthiocerol dimycocerosates, and phenolic glycolipids, belong to the "family" of polyketides, secondary metabolites synthesized by fascinating versatile enzymes-polyketide synthases. These megasynthases consist of multiple catalytic domains, among which the acyltransferase domain plays a key role in selecting and transferring the substrates required for polyketide extension. Here, we present three new crystal structures of acyltransferase domains of mycobacterial polyketide synthases and, for one of them, provide evidence for the identification of residues determining extender unit specificity. Unravelling the molecular basis for such specificity is of high importance considering the role played by extender units for the final structure of key mycobacterial components. This work provides major advances for the use of mycobacterial polyketide synthases as potential therapeutic targets and, more generally, contributes to the prediction and bioengineering of polyketide synthases with desired specificity.

PubMed: 33237724
DOI: 10.1021/acschembio.0c00772

Experimental method

X-RAY DIFFRACTION (2.8 Å)