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7AFA

Bacterial 30S ribosomal subunit assembly complex state F (head domain)

Summary for 7AFA
Entry DOI10.2210/pdb7afa/pdb
EMDB information11758
Descriptor16SrRNA (head domain of the 30S ribosome), MAGNESIUM ION, ZINC ION, ... (11 entities in total)
Functional Keywordscryo-em, 30s biogenesis, ribosome assembly, rbfa, rsga, yjeq, rimp, ksga, rsma, ribosome
Biological sourceEscherichia coli
More
Total number of polymer chains9
Total formula weight634901.67
Authors
Schedlbauer, A.,Iturrioz, I.,Ochoa-Lizarralde, B.,Kaminishi, T.,Diercks, T.,Capuni, R.,Astigarraga, E.,Gil-Carton, D.,Fucini, P.,Connell, S. (deposition date: 2020-09-19, release date: 2021-07-07, Last modification date: 2024-04-24)
Primary citationSchedlbauer, A.,Iturrioz, I.,Ochoa-Lizarralde, B.,Diercks, T.,Lopez-Alonso, J.P.,Lavin, J.L.,Kaminishi, T.,Capuni, R.,Dhimole, N.,de Astigarraga, E.,Gil-Carton, D.,Fucini, P.,Connell, S.R.
A conserved rRNA switch is central to decoding site maturation on the small ribosomal subunit.
Sci Adv, 7:-, 2021
Cited by
PubMed Abstract: While a structural description of the molecular mechanisms guiding ribosome assembly in eukaryotic systems is emerging, bacteria use an unrelated core set of assembly factors for which high-resolution structural information is still missing. To address this, we used single-particle cryo-electron microscopy to visualize the effects of bacterial ribosome assembly factors RimP, RbfA, RsmA, and RsgA on the conformational landscape of the 30 ribosomal subunit and obtained eight snapshots representing late steps in the folding of the decoding center. Analysis of these structures identifies a conserved secondary structure switch in the 16 ribosomal RNA central to decoding site maturation and suggests both a sequential order of action and molecular mechanisms for the assembly factors in coordinating and controlling this switch. Structural and mechanistic parallels between bacterial and eukaryotic systems indicate common folding features inherent to all ribosomes.
PubMed: 34088665
DOI: 10.1126/sciadv.abf7547
PDB entries with the same primary citation
Experimental method
ELECTRON MICROSCOPY (2.95 Å)
Structure validation

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