7ABW

Crystal structure of siderophore reductase FoxB

Summary for 7ABW

• Entry DOI: 10.2210/pdb7abw/pdb
• Descriptor: PepSY domain-containing protein, PROTOPORPHYRIN IX CONTAINING FE, 3,6,9,12,15,18,21,24-OCTAOXAHEXACOSAN-1-OL, ... (5 entities in total)
• Functional Keywords: membrane protein reductase siderophore, membrane protein
• Biological source: Pseudomonas aeruginosa
• Total number of polymer chains: 2
• Total formula weight: 91698.39

Authors

Josts, I.,Tidow, H. (deposition date: 2020-09-09, release date: 2021-09-01, Last modification date: 2024-06-19)

Primary citation

Josts, I.,Veith, K.,Normant, V.,Schalk, I.J.,Tidow, H.
Structural insights into a novel family of integral membrane siderophore reductases.
Proc.Natl.Acad.Sci.USA, 118:-, 2021

PubMed Abstract: Gram-negative bacteria take up the essential ion Fe as ferric-siderophore complexes through their outer membrane using TonB-dependent transporters. However, the subsequent route through the inner membrane differs across many bacterial species and siderophore chemistries and is not understood in detail. Here, we report the crystal structure of the inner membrane protein FoxB (from ) that is involved in Fe-siderophore uptake. The structure revealed a fold with two tightly bound heme molecules. In combination with in vitro reduction assays and in vivo iron uptake studies, these results establish FoxB as an inner membrane reductase involved in the release of iron from ferrioxamine during Fe-siderophore uptake.

PubMed: 34417315
DOI: 10.1073/pnas.2101952118

Experimental method

X-RAY DIFFRACTION (3.35 Å)