7A9X

Structure of yeast Rmd9p in complex with 16nt target RNA

7A9X の概要

• エントリーDOI: 10.2210/pdb7a9x/pdb
• 関連するPDBエントリー: 7A9W
• 分子名称: Protein RMD9, mitochondrial, RNA (5'-R(P*AP*AP*AP*UP*AP*AP*CP*AP*UP*UP*CP*UP*U)-3'), CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: mitochondria, translation, transcription, degradation, ribosome, rna binding protein
• 由来する生物種: Saccharomyces cerevisiae S288C; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 75462.62

構造登録者

Hillen, H.S.,Markov, D.A.,Ireneusz, W.D.,Hofmann, K.B.,Cowan, A.T.,Jones, J.L.,Temiakov, D.,Cramer, P.,Anikin, M. (登録日: 2020-09-02, 公開日: 2021-04-07, 最終更新日: 2024-11-13)

主引用文献

Hillen, H.S.,Markov, D.A.,Wojtas, I.D.,Hofmann, K.B.,Lidschreiber, M.,Cowan, A.T.,Jones, J.L.,Temiakov, D.,Cramer, P.,Anikin, M.
The pentatricopeptide repeat protein Rmd9 recognizes the dodecameric element in the 3'-UTRs of yeast mitochondrial mRNAs.
Proc.Natl.Acad.Sci.USA, 118:-, 2021

PubMed Abstract: Stabilization of messenger RNA is an important step in posttranscriptional gene regulation. In the nucleus and cytoplasm of eukaryotic cells it is generally achieved by 5' capping and 3' polyadenylation, whereas additional mechanisms exist in bacteria and organelles. The mitochondrial mRNAs in the yeast comprise a dodecamer sequence element that confers RNA stability and 3'-end processing via an unknown mechanism. Here, we isolated the protein that binds the dodecamer and identified it as Rmd9, a factor that is known to stabilize yeast mitochondrial RNA. We show that Rmd9 associates with mRNA around dodecamer elements in vivo and that recombinant Rmd9 specifically binds the element in vitro. The crystal structure of Rmd9 bound to its dodecamer target reveals that Rmd9 belongs to the family of pentatricopeptide (PPR) proteins and uses a previously unobserved mode of specific RNA recognition. Rmd9 protects RNA from degradation by the mitochondrial 3'-exoribonuclease complex mtEXO in vitro, indicating that recognition and binding of the dodecamer element by Rmd9 confers stability to yeast mitochondrial mRNAs.

PubMed: 33876744
DOI: 10.1073/pnas.2009329118

実験手法

X-RAY DIFFRACTION (2.45 Å)