7A5P

Human C Complex Spliceosome - Medium-resolution PERIPHERY

7A5P の概要

• エントリーDOI: 10.2210/pdb7a5p/pdb
• EMDBエントリー: 11570 11571 11572 11573 11574
• 分子名称: U2 snRNA, Cell division cycle 5-like protein, Pre-mRNA-splicing factor SYF1, ... (38 entities in total)
• 機能のキーワード: human c complex, spliceosome, hc, human step 1 spliceosome, splicing
• 由来する生物種: Homo sapiens; 詳細
• タンパク質・核酸の鎖数: 48
• 化学式量合計: 3093739.62

構造登録者

Bertram, K.,Kastner, B. (登録日: 2020-08-21, 公開日: 2020-10-14, 最終更新日: 2024-10-09)

主引用文献

Bertram, K.,El Ayoubi, L.,Dybkov, O.,Agafonov, D.E.,Will, C.L.,Hartmuth, K.,Urlaub, H.,Kastner, B.,Stark, H.,Luhrmann, R.
Structural Insights into the Roles of Metazoan-Specific Splicing Factors in the Human Step 1 Spliceosome.
Mol.Cell, 80:127-139.e6, 2020

PubMed Abstract: Human spliceosomes contain numerous proteins absent in yeast, whose functions remain largely unknown. Here we report a 3D cryo-EM structure of the human spliceosomal C complex at 3.4 Å core resolution and 4.5-5.7 Å at its periphery, and aided by protein crosslinking we determine its molecular architecture. Our structure provides additional insights into the spliceosome's architecture between the catalytic steps of splicing, and how proteins aid formation of the spliceosome's catalytically active RNP (ribonucleoprotein) conformation. It reveals the spatial organization of the metazoan-specific proteins PPWD1, WDR70, FRG1, and CIR1 in human C complexes, indicating they stabilize functionally important protein domains and RNA structures rearranged/repositioned during the B to C transition. Structural comparisons with human B, C, and P complexes reveal an intricate cascade of RNP rearrangements during splicing catalysis, with intermediate RNP conformations not found in yeast, and additionally elucidate the structural basis for the sequential recruitment of metazoan-specific spliceosomal proteins.

PubMed: 33007253
DOI: 10.1016/j.molcel.2020.09.012

実験手法

ELECTRON MICROSCOPY (5 Å)