7ZIM

JC Polyomavirus VP1 in complex with 3'-Sialyllactose glycomacromolecules (aromatic linker)

This is a non-PDB format compatible entry.

Summary for 7ZIM

• Entry DOI: 10.2210/pdb7zim/pdb
• Related PRD ID: PRD_002415
• Descriptor: Major capsid protein VP1, N-acetyl-alpha-neuraminic acid-(2-3)-beta-D-galactopyranose-(1-4)-beta-D-glucopyranose, N-acetyl-alpha-neuraminic acid-(2-3)-beta-D-galactopyranose, ... (5 entities in total)
• Functional Keywords: complex, sialic acid, capsid protein, viral protein
• Biological source: JC polyomavirus
• Total number of polymer chains: 5
• Total formula weight: 152330.06

Authors

Freytag, J.,Mueller, J.C.,Stehle, T. (deposition date: 2022-04-08, release date: 2022-12-21, Last modification date: 2024-01-31)

Primary citation

Konietzny, P.B.,Freytag, J.,Feldhof, M.I.,Muller, J.C.,Ohl, D.,Stehle, T.,Hartmann, L.
Synthesis of Homo- and Heteromultivalent Fucosylated and Sialylated Oligosaccharide Conjugates via Preactivated N -Methyloxyamine Precision Macromolecules and Their Binding to Polyomavirus Capsid Proteins.
Biomacromolecules, 23:5273-5284, 2022

PubMed Abstract: Glycoconjugates are a versatile class of bioactive molecules that have found application as vaccines and antivirals and in cancer therapy. Their synthesis typically involves elaborate functionalization and use of protecting groups on the carbohydrate component in order to ensure efficient and selective conjugation. Alternatively, non-functionalized, non-protected carbohydrates isolated from biological sources or derived through biotechnological methods can be directly conjugated -methyloxyamine groups. In this study, we introduce such -methyloxyamine groups into a variety of multivalent scaffolds─from small to oligomeric to polymeric scaffolds─making use of solid-phase polymer synthesis to assemble monodisperse sequence-defined macromolecules. These scaffolds are then successfully functionalized with different types of human milk oligosaccharides deriving a library of homo- and heteromultivalent glycoconjugates. Glycomacromolecules presenting oligosaccharide side chains with either α2,3- or α2,6-linked terminal sialic acid are used in a binding study with two types of polyomavirus capsid proteins showing that the multivalent presentation through the -methyloxyamine-derived scaffolds increases the number of contacts with the protein. Overall, a straightforward route to derive glycoconjugates from complex oligosaccharides with high variability yet control in the multivalent scaffold is presented, and applicability of the derived structures is demonstrated.

PubMed: 36398945
DOI: 10.1021/acs.biomac.2c01092

Experimental method

X-RAY DIFFRACTION (1.55 Å)