7ZIM
JC Polyomavirus VP1 in complex with 3'-Sialyllactose glycomacromolecules (aromatic linker)
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2022-02-05 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 0.99 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 150.146, 96.635, 128.555 |
Unit cell angles | 90.00, 110.49, 90.00 |
Refinement procedure
Resolution | 44.800 - 1.550 |
Rwork | 0.167 |
R-free | 0.19090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3nxd |
RMSD bond length | 0.008 |
RMSD bond angle | 1.502 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.800 | 1.640 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmeas | 0.100 | 1.300 |
Number of reflections | 248912 | 39897 |
<I/σ(I)> | 10.61 | |
Completeness [%] | 95.9 | |
Redundancy | 4.74 | |
CC(1/2) | 0.990 | 0.490 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M HEPES pH 7.5, 0.2 M KSCN, 12% (w/v) PEG 3350 |