Summary for 7Y5E
| Entry DOI | 10.2210/pdb7y5e/pdb |
| EMDB information | 33558 33561 33597 33605 33618 |
| Descriptor | Phycoerythrin alpha subunit, C-phycocyanin beta subunit, (2S)-2,3-dihydroxypropyl octadecanoate, ... (104 entities in total) |
| Functional Keywords | complex, photosynthesis |
| Biological source | Porphyridium purpureum More |
| Total number of polymer chains | 894 |
| Total formula weight | 18653194.67 |
| Authors | You, X.,Zhang, X.,Cheng, J.,Xiao, Y.N.,Sui, S.F. (deposition date: 2022-06-17, release date: 2023-02-01, Last modification date: 2024-07-03) |
| Primary citation | You, X.,Zhang, X.,Cheng, J.,Xiao, Y.,Ma, J.,Sun, S.,Zhang, X.,Wang, H.W.,Sui, S.F. In situ structure of the red algal phycobilisome-PSII-PSI-LHC megacomplex. Nature, 616:199-206, 2023 Cited by PubMed Abstract: In oxygenic photosynthetic organisms, light energy is captured by antenna systems and transferred to photosystem II (PSII) and photosystem I (PSI) to drive photosynthesis. The antenna systems of red algae consist of soluble phycobilisomes (PBSs) and transmembrane light-harvesting complexes (LHCs). Excitation energy transfer pathways from PBS to photosystems remain unclear owing to the lack of structural information. Here we present in situ structures of PBS-PSII-PSI-LHC megacomplexes from the red alga Porphyridium purpureum at near-atomic resolution using cryogenic electron tomography and in situ single-particle analysis, providing interaction details between PBS, PSII and PSI. The structures reveal several unidentified and incomplete proteins and their roles in the assembly of the megacomplex, as well as a huge and sophisticated pigment network. This work provides a solid structural basis for unravelling the mechanisms of PBS-PSII-PSI-LHC megacomplex assembly, efficient energy transfer from PBS to the two photosystems, and regulation of energy distribution between PSII and PSI. PubMed: 36922595DOI: 10.1038/s41586-023-05831-0 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.3 Å) |
Structure validation
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