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- EMDB-33605: PBS of PBS-PSII-PSI-LHCs from Porphyridium purpureum. -

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Basic information

Entry
Database: EMDB / ID: EMD-33605
TitlePBS of PBS-PSII-PSI-LHCs from Porphyridium purpureum.
Map data
Sample
  • Cell: In situ PBS from Porphyridium purpureum
    • Protein or peptide: x 30 types
  • Ligand: x 3 types
Function / homology
Function and homology information


phycobilisome / chloroplast thylakoid membrane / photosynthesis / lyase activity
Similarity search - Function
Allophycocyanin linker chain / FAS1 domain / FAS1 domain superfamily / Fasciclin domain / FAS1/BIgH3 domain profile. / Four repeated domains in the Fasciclin I family of proteins, present in many other contexts. / Phycobilisome linker protein / Allophycocyanin, beta subunit / Phycobilisome linker domain / Phycobilisome linker domain superfamily ...Allophycocyanin linker chain / FAS1 domain / FAS1 domain superfamily / Fasciclin domain / FAS1/BIgH3 domain profile. / Four repeated domains in the Fasciclin I family of proteins, present in many other contexts. / Phycobilisome linker protein / Allophycocyanin, beta subunit / Phycobilisome linker domain / Phycobilisome linker domain superfamily / Phycobilisome Linker polypeptide / Phycobilisome (PBS) linker domain profile. / Phycocyanin, alpha subunit / Phycocyanin, beta subunit / CpcD-like domain / CpcD/allophycocyanin linker domain / CpcD-like domain profile. / CpcD/allophycocyanin linker domain / Phycobilisome, alpha/beta subunit / Phycobilisome, alpha/beta subunit superfamily / Phycobilisome protein / Globin-like superfamily
Similarity search - Domain/homology
Phycobiliprotein ApcE / Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod / Uncharacterized protein / Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod / Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod / Uncharacterized protein / R-phycoerythrin gamma chain, chloroplastic / Uncharacterized protein / Phycobilisome 7.8 kDa linker polypeptide, allophycocyanin-associated, core / R-phycoerythrin gamma chain, chloroplastic ...Phycobiliprotein ApcE / Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod / Uncharacterized protein / Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod / Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod / Uncharacterized protein / R-phycoerythrin gamma chain, chloroplastic / Uncharacterized protein / Phycobilisome 7.8 kDa linker polypeptide, allophycocyanin-associated, core / R-phycoerythrin gamma chain, chloroplastic / Phycobilisome 32.1 kDa linker polypeptide, phycocyanin-associated, rod / Uncharacterized protein / Uncharacterized protein / R-phycoerythrin gamma chain, chloroplastic / R-phycoerythrin gamma chain, chloroplastic / FAS1 domain-containing protein / Phycobilisome 27.9 kDa linker polypeptide, phycoerythrin-associated, rod / Uncharacterized protein / Phycobilisome 27.9 kDa linker polypeptide, phycoerythrin-associated, rod / Uncharacterized protein / Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod / FAS1 domain-containing protein / Phycoerythrin alpha subunit / B-phycoerythrin beta chain / C-phycocyanin alpha subunit / Allophycocyanin alpha subunit / Phycobilisome rod-core linker polypeptide / C-phycocyanin beta subunit / Allophycocyanin beta 18 subunit / Allophycocyanin gamma subunit / Allophycocyanin beta subunit
Similarity search - Component
Biological speciesPorphyridium purpureum (eukaryote)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.3 Å
AuthorsYou X / Zhang X / Cheng J / Xiao YN / Sun S / Sui SF
Funding support1 items
OrganizationGrant numberCountry
Not funded
CitationJournal: Nature / Year: 2023
Title: In situ structure of the red algal phycobilisome-PSII-PSI-LHC megacomplex.
Authors: Xin You / Xing Zhang / Jing Cheng / Yanan Xiao / Jianfei Ma / Shan Sun / Xinzheng Zhang / Hong-Wei Wang / Sen-Fang Sui /
Abstract: In oxygenic photosynthetic organisms, light energy is captured by antenna systems and transferred to photosystem II (PSII) and photosystem I (PSI) to drive photosynthesis. The antenna systems of red ...In oxygenic photosynthetic organisms, light energy is captured by antenna systems and transferred to photosystem II (PSII) and photosystem I (PSI) to drive photosynthesis. The antenna systems of red algae consist of soluble phycobilisomes (PBSs) and transmembrane light-harvesting complexes (LHCs). Excitation energy transfer pathways from PBS to photosystems remain unclear owing to the lack of structural information. Here we present in situ structures of PBS-PSII-PSI-LHC megacomplexes from the red alga Porphyridium purpureum at near-atomic resolution using cryogenic electron tomography and in situ single-particle analysis, providing interaction details between PBS, PSII and PSI. The structures reveal several unidentified and incomplete proteins and their roles in the assembly of the megacomplex, as well as a huge and sophisticated pigment network. This work provides a solid structural basis for unravelling the mechanisms of PBS-PSII-PSI-LHC megacomplex assembly, efficient energy transfer from PBS to the two photosystems, and regulation of energy distribution between PSII and PSI.
History
DepositionJun 15, 2022-
Header (metadata) releaseJan 18, 2023-
Map releaseJan 18, 2023-
UpdateApr 19, 2023-
Current statusApr 19, 2023Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_33605.png

Downloads & links

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Map

FileDownload / File: emd_33605.map.gz / Format: CCP4 / Size: 216 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.632 Å
Density
Contour LevelBy AUTHOR: 0.36
Minimum - Maximum-0.8327662 - 1.3533211
Average (Standard dev.)0.0018886954 (±0.08101401)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions384384384
Spacing384384384
CellA=B=C: 626.688 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_33605_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_33605_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
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Histogram

Histogram (log scale)

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Sample components

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Entire : In situ PBS from Porphyridium purpureum

EntireName: In situ PBS from Porphyridium purpureum
Components
  • Cell: In situ PBS from Porphyridium purpureum
    • Protein or peptide: Linker4
    • Protein or peptide: B-phycoerythrin beta chain
    • Protein or peptide: Phycoerythrin alpha subunit
    • Protein or peptide: CaRSPs1
    • Protein or peptide: CaRSPs2
    • Protein or peptide: R-phycoerythrin gamma chain, chloroplastic
    • Protein or peptide: Phycobilisome rod-core linker polypeptide
    • Protein or peptide: C-phycocyanin alpha subunit
    • Protein or peptide: C-phycocyanin beta subunit
    • Protein or peptide: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod
    • Protein or peptide: Phycobilisome 27.9 kDa linker polypeptide, phycoerythrin-associated, rod
    • Protein or peptide: R-phycoerythrin gamma chain, chloroplastic
    • Protein or peptide: R-phycoerythrin gamma chain, chloroplastic
    • Protein or peptide: R-phycoerythrin gamma chain, chloroplastic
    • Protein or peptide: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod
    • Protein or peptide: Lrc4
    • Protein or peptide: LRC5
    • Protein or peptide: FAS1 domain-containing protein
    • Protein or peptide: Allophycocyanin alpha subunit
    • Protein or peptide: Allophycocyanin beta subunit
    • Protein or peptide: Allophycocyanin gamma subunit
    • Protein or peptide: Allophycocyanin beta 18 subunit
    • Protein or peptide: Phycobilisome 7.8 kDa linker polypeptide, allophycocyanin-associated, core
    • Protein or peptide: Phycobiliprotein ApcE
    • Protein or peptide: Phycobilisome 32.1 kDa linker polypeptide, phycocyanin-associated, rod
    • Protein or peptide: Phycobilisome 27.9 kDa linker polypeptide, phycoerythrin-associated, rod
    • Protein or peptide: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod
    • Protein or peptide: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod
    • Protein or peptide: FAS1 domain-containing protein
    • Protein or peptide: LPP2
  • Ligand: PHYCOERYTHROBILIN
  • Ligand: PHYCOUROBILIN
  • Ligand: PHYCOCYANOBILIN

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Supramolecule #1: In situ PBS from Porphyridium purpureum

SupramoleculeName: In situ PBS from Porphyridium purpureum / type: cell / ID: 1 / Parent: 0 / Macromolecule list: #1-#30
Source (natural)Organism: Porphyridium purpureum (eukaryote)

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Macromolecule #1: Linker4

MacromoleculeName: Linker4 / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 14.749816 KDa
Recombinant expressionOrganism: Porphyridium purpureum (eukaryote)
SequenceString:
MAFVTGGLVG SSSAPALRTV CNASQSKLRM AASAADVVNA AYPKNIKNKA PVISFDGKKG VKLEMVTLQT FAGDDSEDTL FDYSSGKFM PQKPADMGIA WPSGDGRQAE MKGGKGSFNQ PDLRKYGPFP DFLKRSMDL

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Macromolecule #2: B-phycoerythrin beta chain

MacromoleculeName: B-phycoerythrin beta chain / type: protein_or_peptide / ID: 2 / Number of copies: 276 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 18.584182 KDa
Recombinant expressionOrganism: Porphyridium purpureum (eukaryote)
SequenceString:
MLDAFSRVVV NSDAKAAYVG GSDLQALKSF IADGNKRLDA VNSIVSNASC MVSDAVSGMI CENPGLISPG G(MEN)CYTN RRM AACLRDGEII LRYVSYALLA GDASVLEDRC LNGLKETYIA LGVPTNSSIR AVSIMKAQAV AFITNTATER KMSFAAG DC TSLASEVASY FDRVGAAIS

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Macromolecule #3: Phycoerythrin alpha subunit

MacromoleculeName: Phycoerythrin alpha subunit / type: protein_or_peptide / ID: 3 / Number of copies: 254 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 17.824029 KDa
Recombinant expressionOrganism: Porphyridium purpureum (eukaryote)
SequenceString:
MKSVITTVVS AADAAGRFPS NSDLESIQGN IQRSAARLEA AEKLAGNHEA VVKEAGDACF AKYAYLKNPG EAGENQEKIN KCYRDVDHY MRLVNYCLVV GGTGPLDEWG IAGAREVYRT LNLPTSAYVA SIAYTRDRLC VPRDMSAQAG VEFSAYLDYL I NALS

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Macromolecule #4: CaRSPs1

MacromoleculeName: CaRSPs1 / type: protein_or_peptide / ID: 4 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 28.151357 KDa
Recombinant expressionOrganism: Porphyridium purpureum (eukaryote)
SequenceString: MAAFVSGGCG VGGQRRAWPA KGAAVARTHA CPTTMVVLPV ARAGLAATAK KNQYMGTSVA PEIVLTDKGS DMSRKVKTED KKVAADQAA AMGILANMSL YASLNPVKRM TYKAKEQAPA YVKKTGNPVE DFYPSSWRNM APVISLSANR VAVAFEKIDA A SNGVKANS ...String:
MAAFVSGGCG VGGQRRAWPA KGAAVARTHA CPTTMVVLPV ARAGLAATAK KNQYMGTSVA PEIVLTDKGS DMSRKVKTED KKVAADQAA AMGILANMSL YASLNPVKRM TYKAKEQAPA YVKKTGNPVE DFYPSSWRNM APVISLSANR VAVAFEKIDA A SNGVKANS NNKPFWKSGA TTKNYVAPEA PAQSEPETLD DAAYQRYFPA RIRNKAPAME FRRPSFANTE DPSAYFMLQK ET VPLRMAL AEKLLTKLGR KGDG

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Macromolecule #5: CaRSPs2

MacromoleculeName: CaRSPs2 / type: protein_or_peptide / ID: 5 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 35.163527 KDa
Recombinant expressionOrganism: Porphyridium purpureum (eukaryote)
SequenceString: MWEQQRPRRC EAPAAPSSRP AERRAAARRS RAQLRMKQDD YEQWKTEFAG GFPGGEAFYK KWIEEGAKGD VPALEEELQP RSPNKKPTI YEEQMISNRG QQKGVDPTWK TLLAGGFPGG EFFFKKWIGE GAQGEVPNLD ADLQPGSGSA KKTGKKEDAD K SSPGGIMT ...String:
MWEQQRPRRC EAPAAPSSRP AERRAAARRS RAQLRMKQDD YEQWKTEFAG GFPGGEAFYK KWIEEGAKGD VPALEEELQP RSPNKKPTI YEEQMISNRG QQKGVDPTWK TLLAGGFPGG EFFFKKWIGE GAQGEVPNLD ADLQPGSGSA KKTGKKEDAD K SSPGGIMT PGRIMVPSGL GEEEETVDKE APRPELYNKY FSADRLHKAP EILFEYNKTK YDRVGVRYTE VTSKASERFF PK SRMNRAP VIEISYREGA VSTASVSLSM PEISGPPALP FPVPKGDVTT TMVTDPTTGR LKLEFKVDGA AVSAYSDPRA

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Macromolecule #6: R-phycoerythrin gamma chain, chloroplastic

MacromoleculeName: R-phycoerythrin gamma chain, chloroplastic / type: protein_or_peptide / ID: 6 / Number of copies: 10 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 31.792982 KDa
Recombinant expressionOrganism: Porphyridium purpureum (eukaryote)
SequenceString: MAAFVSGFHG VQVGAPAENK LVCRAAKPAQ LTMLTGYDSK SSPNFPNRAA TRERRTVSFN ARVARNKSQA KKILEKADEF FARSVTMQY KAFACPNGVY DIQCTEGTVK GAAYEKRAMA VSAAFRAKQA SPAAKARALF ENRRHAIIAS HECQHEEDLF V RFPKLSAA ...String:
MAAFVSGFHG VQVGAPAENK LVCRAAKPAQ LTMLTGYDSK SSPNFPNRAA TRERRTVSFN ARVARNKSQA KKILEKADEF FARSVTMQY KAFACPNGVY DIQCTEGTVK GAAYEKRAMA VSAAFRAKQA SPAAKARALF ENRRHAIIAS HECQHEEDLF V RFPKLSAA YMMGKTEAMR TCSRYVVPDS LEEEYMAASV DRQMKERACP GGVYASSCVE GNAKGQAEQA RVAALATAFR SA QKSASKT TAERYSSAAY GRDHFAHGCS YEESVFNTYP ATAAAMRSKS YNY

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Macromolecule #7: Phycobilisome rod-core linker polypeptide

MacromoleculeName: Phycobilisome rod-core linker polypeptide / type: protein_or_peptide / ID: 7 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 26.996689 KDa
Recombinant expressionOrganism: Porphyridium purpureum (eukaryote)
SequenceString: MSIPILKYSL STQNQRVESF EVNPGDEQYR IYSTDSLPSS SEVDQIIWAA YRQIFSEHQI LKSTKQPFLE SQLRFNQISI KDFIKGLVL SAPFRDLNYN VNNNYRFVEI CVQRVLGRDI YNEREKIAWS IIIATQGLEG FIDTLLNTQE YLSNFGDSIV P FQRRRIIP ...String:
MSIPILKYSL STQNQRVESF EVNPGDEQYR IYSTDSLPSS SEVDQIIWAA YRQIFSEHQI LKSTKQPFLE SQLRFNQISI KDFIKGLVL SAPFRDLNYN VNNNYRFVEI CVQRVLGRDI YNEREKIAWS IIIATQGLEG FIDTLLNTQE YLSNFGDSIV P FQRRRIIP QRSKGEMPFN LKTPRYGIEF RSKLAATQFS WASPIRRFRP QEQQPKAGDP ALFLNMVQEM GPGL

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Macromolecule #8: C-phycocyanin alpha subunit

MacromoleculeName: C-phycocyanin alpha subunit / type: protein_or_peptide / ID: 8 / Number of copies: 36 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 17.478664 KDa
Recombinant expressionOrganism: Porphyridium purpureum (eukaryote)
SequenceString:
MKTPITEAIA TADNQGRFLS NTELQAVNGR YQRATASLTA AKALTGSAQR LITGAAQAVY NKFPYTTQMP GPAYASSAIG KAKCARDIG YYLRMVTYCL VVGGTGPMDE YLVAGLEEIN RSFDLSPSWY IEALQYIKNS HGLSGQVANE ANAYIDYAIN T LS

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Macromolecule #9: C-phycocyanin beta subunit

MacromoleculeName: C-phycocyanin beta subunit / type: protein_or_peptide / ID: 9 / Number of copies: 36 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 18.242705 KDa
Recombinant expressionOrganism: Porphyridium purpureum (eukaryote)
SequenceString:
MLDAFAKVVA QADARGEFLS NTQIDALSKM VKEGNQRLDI VNKVTSNASA IVTNSARALF AEQPQLIQPG G(MEN)AYTS RRM AACLRDMEIV LRYVSYAMIA GDSSVLDDRC LNGLRETYQA LGTPGSSVAV AIQKMKDASV ALANDTTGTP IGDCSSL VA ELAGYFDRAA VSVV

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Macromolecule #10: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associat...

MacromoleculeName: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod
type: protein_or_peptide / ID: 10 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 39.727625 KDa
Recombinant expressionOrganism: Porphyridium purpureum (eukaryote)
SequenceString: MAAFVTGMGA APRSQMRSVS GSAVCVRSDS VRAPGALTMR LGTVSGNSSL DKLGLDKFLS ESNRAYTPRA QPGFSSEYEQ IISATYKQL FGNAYIMDSE RAEMAKQESM FRDGQLTLKD FCRALAKTEQ YKKRFFDSRP LYGAIELNFK NILGRTPDGL E HYRAKSAV ...String:
MAAFVTGMGA APRSQMRSVS GSAVCVRSDS VRAPGALTMR LGTVSGNSSL DKLGLDKFLS ESNRAYTPRA QPGFSSEYEQ IISATYKQL FGNAYIMDSE RAEMAKQESM FRDGQLTLKD FCRALAKTEQ YKKRFFDSRP LYGAIELNFK NILGRTPDGL E HYRAKSAV YDTKGYEAFV DAFFDDGEYD EVYDDYTVPF YRGYKTEANL SMAAFTHFFR MVRGSSTSDK ANPNSMQKDI PL NYYGITK TPLAVIAPGA AGTAYTESFA GTGSWQSGRA GLNAARVALG VPATANGKSF RVEVTGYTQP GFGITAGTAV GKL YKANKL SRYPRSNKSY VVGFDELTPL YQRITKNGGT IASITPL

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Macromolecule #11: Phycobilisome 27.9 kDa linker polypeptide, phycoerythrin-associat...

MacromoleculeName: Phycobilisome 27.9 kDa linker polypeptide, phycoerythrin-associated, rod
type: protein_or_peptide / ID: 11 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 34.958746 KDa
Recombinant expressionOrganism: Porphyridium purpureum (eukaryote)
SequenceString: MAFVSGAGAA VRGTSGKAAA RCAAVRTVRA ARAIRMTTAP GSAPFSSLDE PERQVVMWES VGDEKDQVFK QLYRQVFGNA YLMESDLEE LLVPESQLLM GSISVKDFIK RVAKSDAYKK RFFEPCGPYR FVELCTKHFL GRGPRDQKEV SEHVQRLANE G YDADVDSY ...String:
MAFVSGAGAA VRGTSGKAAA RCAAVRTVRA ARAIRMTTAP GSAPFSSLDE PERQVVMWES VGDEKDQVFK QLYRQVFGNA YLMESDLEE LLVPESQLLM GSISVKDFIK RVAKSDAYKK RFFEPCGPYR FVELCTKHFL GRGPRDQKEV SEHVQRLANE G YDADVDSY MDSEEYMSLF GENGVPRFVF KGTYEGNDQF NRLAAMRQFA DGSYTDTRSG STAPRKAQKA ELTMAEGDFV GR AKVSRGL PAETSAAKTG TPPVRALKGP VNPRAGVRVR IKVVDNLYQV YEIPPMADPK AKVNAFWAKP IPSSLTKKY

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Macromolecule #12: R-phycoerythrin gamma chain, chloroplastic

MacromoleculeName: R-phycoerythrin gamma chain, chloroplastic / type: protein_or_peptide / ID: 12 / Number of copies: 6 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 29.453639 KDa
Recombinant expressionOrganism: Porphyridium purpureum (eukaryote)
SequenceString: MAAFVGSAAS AFTGASAVKA NEKRSVCSLQ MVAMPQTGLV NSKFSARMAK KTAKQTKNKV DEYMARSVQR QYKQAAVATG VYGTQCTEG TVKGAAEASR SAALSRQFRI KQRSAFSKAH DLFEFRKHAI IAAAGCSYEE KMVTRFPKLA AAMVLGQTEM M RTCSRYVV ...String:
MAAFVGSAAS AFTGASAVKA NEKRSVCSLQ MVAMPQTGLV NSKFSARMAK KTAKQTKNKV DEYMARSVQR QYKQAAVATG VYGTQCTEG TVKGAAEASR SAALSRQFRI KQRSAFSKAH DLFEFRKHAI IAAAGCSYEE KMVTRFPKLA AAMVLGQTEM M RTCSRYVV PESVEEEYMA ASVDKQMKRR GAPGGVYSLS CAEGVAKGQA EIARVSALGA AYRAASKSAS AVTAERYNSM AY GRVHFAH GCSYEEQQFN KYPAAAAAMR SDSYGY

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Macromolecule #13: R-phycoerythrin gamma chain, chloroplastic

MacromoleculeName: R-phycoerythrin gamma chain, chloroplastic / type: protein_or_peptide / ID: 13 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 31.358787 KDa
Recombinant expressionOrganism: Porphyridium purpureum (eukaryote)
SequenceString: METAFVSGFM GKAAVAKFGA TAVCDKTARR SSSSNSQVHM VTGAVSSVNM RRFQRVPKVS GFSAKVTKKN VNKALDKADM FFAKSVTME GKAAAIPYGV YGIQCMEGSA KGMAHEKRAM ALSAAFRMNQ RSAAEKTGAM YENRRLALIL AQNDHQEKQY I KYPKLAAA ...String:
METAFVSGFM GKAAVAKFGA TAVCDKTARR SSSSNSQVHM VTGAVSSVNM RRFQRVPKVS GFSAKVTKKN VNKALDKADM FFAKSVTME GKAAAIPYGV YGIQCMEGSA KGMAHEKRAM ALSAAFRMNQ RSAAEKTGAM YENRRLALIL AQNDHQEKQY I KYPKLAAA ALMASTEVTR ACQRYAVPES IEEEFLAASV DKVNKMRGTT ASGVYKSSCV EGNAKGQAEQ ARVAALAVAF RS AQKSASQ FAAERYAQSK YGRDLFSSTH FEEGYANTYP AMAAAKRASS YGY

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Macromolecule #14: R-phycoerythrin gamma chain, chloroplastic

MacromoleculeName: R-phycoerythrin gamma chain, chloroplastic / type: protein_or_peptide / ID: 14 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 36.924992 KDa
Recombinant expressionOrganism: Porphyridium purpureum (eukaryote)
SequenceString: MAAFVVGAQV SSGFMGCAAP RKEQTRVGGA AKAAVSMRII IQDKNSYRKY QTNTSTSKWD KLLNTKPMKR QVQPNPPTNE TRALNLGNT FRSPAFKFLG TLKRSKDPSG LRLGFYGRKA DDFMARSIAM QAKASAAGSG VYTTQCSEGA SKGMAENART A SLAKQFRQ ...String:
MAAFVVGAQV SSGFMGCAAP RKEQTRVGGA AKAAVSMRII IQDKNSYRKY QTNTSTSKWD KLLNTKPMKR QVQPNPPTNE TRALNLGNT FRSPAFKFLG TLKRSKDPSG LRLGFYGRKA DDFMARSIAM QAKASAAGSG VYTTQCSEGA SKGMAENART A SLAKQFRQ AQRSAREMSF DYYEGRKYAM KAVGHICNYE EKIFQQYNKT AAAYVMGKQE TLLSCDRYAQ PANKAEEYIQ KS VQMQMKK RSIPYGVYTT SCADGTVKGM AENARVAKES ANFRARQMSA GAKAAARFNA RRVANDWHNN GCNYEEKLTS RFP AAASSV RPTTNRY

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Macromolecule #15: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associat...

MacromoleculeName: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod
type: protein_or_peptide / ID: 15 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 32.886242 KDa
Recombinant expressionOrganism: Porphyridium purpureum (eukaryote)
SequenceString: MEAVGFVHVG AWRVGSSSAA AAAAGVAGKR CGALPVSFGA HAPRRAASAS QVKMMAFSSI QNVLTDAPLG TPSAMGFLDE RTEKPLMGP SEMDELVYAV YKQVFGNAHI MESERAELRD AESLFRYLRD VREFVRALAK SNAYTSRFLQ GNQQYRFFEL N FKHLLGRG ...String:
MEAVGFVHVG AWRVGSSSAA AAAAGVAGKR CGALPVSFGA HAPRRAASAS QVKMMAFSSI QNVLTDAPLG TPSAMGFLDE RTEKPLMGP SEMDELVYAV YKQVFGNAHI MESERAELRD AESLFRYLRD VREFVRALAK SNAYTSRFLQ GNQQYRFFEL N FKHLLGRG PRSLEEMASH SALYAEGGFE AVIDAILDSD EYAQEFGSTV VPYCRFKGEY PTNEEFNRML VLKGANSTSD KA GKGRSKL CYSIAAGCSQ NWLSMSKALP FGTEKGTGFN LISSSRNPAA RPRIGTKIPG GVVFSS

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Macromolecule #16: Lrc4

MacromoleculeName: Lrc4 / type: protein_or_peptide / ID: 16 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 16.91526 KDa
Recombinant expressionOrganism: Porphyridium purpureum (eukaryote)
SequenceString:
MAFVACGPLR AGEGGARLGA RKAACSMQLA PPGIPPGEDA RNNQSLRQYV ARPVETYQKR SFATPLPLTW TGETETVGAF DVVVPPQEK DLPVSGEATS AFVKYSDMVR AERKAALQAL LSASAAGEGR PTCGAEGRKF VSNANPVLVN GVKCVEYWRK

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Macromolecule #17: LRC5

MacromoleculeName: LRC5 / type: protein_or_peptide / ID: 17 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 30.272959 KDa
Recombinant expressionOrganism: Porphyridium purpureum (eukaryote)
SequenceString: MAFVSGAGVA VPAGAKASAP LCALRMSGYG DYSYSTDRTK GHVNQYYVDK ARSRSDWGNR NVLPASEGDA VLGRTAKGAV AVPEFGIPQ LDDPVLGFGP DSMVDPRIAE ADGAVWRWDA GFVDESMTLA SCADISDEAV ADEAFAKFRG SVLAERGAMI T KAESATAS ...String:
MAFVSGAGVA VPAGAKASAP LCALRMSGYG DYSYSTDRTK GHVNQYYVDK ARSRSDWGNR NVLPASEGDA VLGRTAKGAV AVPEFGIPQ LDDPVLGFGP DSMVDPRIAE ADGAVWRWDA GFVDESMTLA SCADISDEAV ADEAFAKFRG SVLAERGAMI T KAESATAS VITSLRDGLY SGEAQLLTAS GQRLANVAGQ EKIATISGYT WDGQPQTEIP GKPFVKSIGA MDYMDGVEGG DV VAAKVGA FWKPKAPKEV PYKRPMGANT PELPYNTVPR LVQAAGLAVQ E

+
Macromolecule #18: FAS1 domain-containing protein

MacromoleculeName: FAS1 domain-containing protein / type: protein_or_peptide / ID: 18 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 26.620727 KDa
Recombinant expressionOrganism: Porphyridium purpureum (eukaryote)
SequenceString: MAGFVASTGA VLRKQQVDAA PVCERASSRA RAVAPLSMAR TAYPYTGSGY GSAGVPYGQD TYGYKATTAK SITETAAQAG VFNTFVKLL NESGVEKLVE QAGPYTVFAP TDDAFAALLE PHSFNKLATL LRPENNDALR KVLMHHVIPG AFTSASLMDR A VTVKSLAG ...String:
MAGFVASTGA VLRKQQVDAA PVCERASSRA RAVAPLSMAR TAYPYTGSGY GSAGVPYGQD TYGYKATTAK SITETAAQAG VFNTFVKLL NESGVEKLVE QAGPYTVFAP TDDAFAALLE PHSFNKLATL LRPENNDALR KVLMHHVIPG AFTSASLMDR A VTVKSLAG EPISIMGLNK LVTAGTAKVV RADVPCANGC IIHAVSSVII PPNYVPVPQP TKPVFPRSVI AEIAKLPTPR QA LGLDPAP SKIVKY

+
Macromolecule #19: Allophycocyanin alpha subunit

MacromoleculeName: Allophycocyanin alpha subunit / type: protein_or_peptide / ID: 19 / Number of copies: 20 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 17.639123 KDa
Recombinant expressionOrganism: Porphyridium purpureum (eukaryote)
SequenceString:
MSIVTKSIVN ADAEARYLSP GELDRIKSFV LSGQRRLRIA QTLTENRERI VKQGGQQLFQ RRPDVVSPGG NAYGEEMTAT CLRDLDYYL RLVTYGIIAG DVTPIEEIGL VGVKEMYSAL GTPISGVAEG IRCMKDVACS LLSGEDAAEV GFYFDYTLAA M Q

+
Macromolecule #20: Allophycocyanin beta subunit

MacromoleculeName: Allophycocyanin beta subunit / type: protein_or_peptide / ID: 20 / Number of copies: 22 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 17.415797 KDa
Recombinant expressionOrganism: Porphyridium purpureum (eukaryote)
SequenceString:
MQDAITSVIN AADVQGKYLD ANSVEKLRGY FQTGELRVRA AATIAANAAT IIKEAVAKSL LYSDITRPGG (MEN)MYTTR RYA ACIRDLDYYL RYATYGMLAG DPSILDERVL NGLKETYNSL GVPIGATIQA IQAMKEVTGS LVGSDAGKEM GLYFDYI CS GLS

+
Macromolecule #21: Allophycocyanin gamma subunit

MacromoleculeName: Allophycocyanin gamma subunit / type: protein_or_peptide / ID: 21 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 18.126934 KDa
Recombinant expressionOrganism: Porphyridium purpureum (eukaryote)
SequenceString:
MSLVTQVILS ADDELRYPTA GELETISSYL KTGEYRIRLI SILQGKEQEI IRLASKKIFQ LHPEYIAPGG NASGARQRAL CLRDYGWYL RLITYAILAG DKEPLEKIGI IGVREMYNSL GVPIIGMIDA IKCLKEATVE VISQEEEDFV APYYDYIIQG M S

+
Macromolecule #22: Allophycocyanin beta 18 subunit

MacromoleculeName: Allophycocyanin beta 18 subunit / type: protein_or_peptide / ID: 22 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 19.77874 KDa
Recombinant expressionOrganism: Porphyridium purpureum (eukaryote)
SequenceString:
MQDIITSVIN QYDLSGRYLD IKGINQINNY FDTGLKRLMI AKLINKEATN LIKEASELLF IEQPELLRPC GNAFIGNAYT TRRYAACIR DIEYYLRYSA YSIIAGNNSI LDERVLDGLK ETYNSLLVPI GPTIRVIQLL KEIIQKKFST ENIENAIIAE P FDYLAINL SDKNL

+
Macromolecule #23: Phycobilisome 7.8 kDa linker polypeptide, allophycocyanin-associa...

MacromoleculeName: Phycobilisome 7.8 kDa linker polypeptide, allophycocyanin-associated, core
type: protein_or_peptide / ID: 23 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 10.428983 KDa
Recombinant expressionOrganism: Porphyridium purpureum (eukaryote)
SequenceString:
MAYDMDGTGA GSLEPRGIKS SAVEKDSRCF KVTFVLPSQS RLYTFRELQN VYTTKMVPFS SWYAEQQRIQ KMGGKILKVE LVAGGQMRS VGNQ

+
Macromolecule #24: Phycobiliprotein ApcE

MacromoleculeName: Phycobiliprotein ApcE / type: protein_or_peptide / ID: 24 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 99.484406 KDa
Recombinant expressionOrganism: Porphyridium purpureum (eukaryote)
SequenceString: MVTKASGGSP VVKPQLYKTA SMLTIAQAEQ QDRFLELGEL NQLVSFLNTG NIRLEIADLL TKNANIIVAR AADRIFVGGS AISYLERPQ ASIIEANSAD IASIRQMSGD SQSNFLENLT SVFQGNNATP TGFKPISVVR YGPSRMKKSL RDLDWFLRYL T YAIVASDP ...String:
MVTKASGGSP VVKPQLYKTA SMLTIAQAEQ QDRFLELGEL NQLVSFLNTG NIRLEIADLL TKNANIIVAR AADRIFVGGS AISYLERPQ ASIIEANSAD IASIRQMSGD SQSNFLENLT SVFQGNNATP TGFKPISVVR YGPSRMKKSL RDLDWFLRYL T YAIVASDP NILFVNIRGL REIIENACSS AATIVALKEM KKTSLSLFPE NSIQKEIIEE YFNVVVDEFI NPALTDTIRK RT SNDLQGL RLPQIYAKAG ISRQKFVMKP GLSTDEKQSV ISACYRQVFE RDISKAYGFS FSVLESQVKN GQISIKEFVR SLG KSSVYQ KQFYQPYVNS RVVELAFRHF LGRNLSSLAE FQKFFAILSK KGLTGLVDSL INSREYSDYF NEETVPYIRG FGEE PQECR NWGTQIDLFQ YSAPFRKVPQ SITLFSDYLK ALPDQHPYGR GNDPLLIQFG AIFPIGTKNL KQNPAPFGKD TRRLL IRRG PGIYNQVGNP STRSVSVGSL GPKVFKSEGI NSNAQKTNNE SILQASYLAV FGRMIYQNER IGLKGIDNKF LDNNLS VKE LIRSLAISDT FRSLYWTPLY VCKSIEWIHY RLLGRPTYGR QEINQYFNIA YKKGFVGVIN SIIDSVEYNE CFGDNIV PY ERYLTANSVS QRQLKLGNII KSANLKPQNI EKFVQLGQSQ TNQNLYSIKY KVKQGVSKLR DQQKIFETKG SLSKDAYL S IFQAACRQIF ERDISTFVIG NEIENIKIQF IKGQISVKEM INALGKSSVY LKEFYNPYPN IKVIELGTKH FLGRAPNNQ AEIRFYNQIL ASCGLQAFID MLTNSQEYAE IFGEVRVPFR RFPTLPAANF PNTNTLFDKQ TKQNSVVIVP SFKAITGNQ

+
Macromolecule #25: Phycobilisome 32.1 kDa linker polypeptide, phycocyanin-associated, rod

MacromoleculeName: Phycobilisome 32.1 kDa linker polypeptide, phycocyanin-associated, rod
type: protein_or_peptide / ID: 25 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 53.601336 KDa
Recombinant expressionOrganism: Porphyridium purpureum (eukaryote)
SequenceString: MAFVNGFSGG AAAVPRRGVA RVCAQRVVMK VVDRLDSQPS AAFEQTKQVY TFSRYILGPH RAVVAPVAMD PSEKEVVLRA VYRQVFGNA YIMEEERAEL RVMESQFLLG ELSVKELVRA LAKSSTYKVR FFEGAVQYRF IELCFKHLLG RAPDNHEEIA V HMRKYQQE ...String:
MAFVNGFSGG AAAVPRRGVA RVCAQRVVMK VVDRLDSQPS AAFEQTKQVY TFSRYILGPH RAVVAPVAMD PSEKEVVLRA VYRQVFGNA YIMEEERAEL RVMESQFLLG ELSVKELVRA LAKSSTYKVR FFEGAVQYRF IELCFKHLLG RAPDNHEEIA V HMRKYQQE GYDAEIDSYL DAGEYDNVFG DDTVPFLRFR GVYTPCDSFN RQCALQGGWA NSDKAMGGAA LSGYNGSDGR QM STMIGNY ISGKPIPYEK VAADTPLKST APNWYARPNP ALAPQPAYVS AKEIAELRSR VSKLEAAWSV AVKQSAAAKD TVE TWRAAA KEMAAMRGIS PMGEAYFGGI AQKVDNGALA QLGNKASSYK KYLYAIETDE VSRLEVDLEE AKGQLRVLEA AMAK STPMT RTAEFKTLTK NVAAVTAAEK ADPLSKRPRI SASRPKPTPA AAKMAAAEKK KGPFGLSVPS LPNLPNLPKL PSLSL PKVS LPFGKK

+
Macromolecule #26: Phycobilisome 27.9 kDa linker polypeptide, phycoerythrin-associat...

MacromoleculeName: Phycobilisome 27.9 kDa linker polypeptide, phycoerythrin-associated, rod
type: protein_or_peptide / ID: 26 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 36.321957 KDa
Recombinant expressionOrganism: Porphyridium purpureum (eukaryote)
SequenceString: MAFVTGGVGA VVRPGTAVSR GVVCARQQAR PRAAALQMGV SDVSITLSGV AASQPVSAPA KMSLEDRQLL VLQAIKQVFG NAYVMEEER ASFAKQESMF LSGELSVREF VRELALSDTY RRRFFEPCGP YRFVELNMKH LLGRGPISQA EVSQHVQCYV N NGYEAEIS ...String:
MAFVTGGVGA VVRPGTAVSR GVVCARQQAR PRAAALQMGV SDVSITLSGV AASQPVSAPA KMSLEDRQLL VLQAIKQVFG NAYVMEEER ASFAKQESMF LSGELSVREF VRELALSDTY RRRFFEPCGP YRFVELNMKH LLGRGPISQA EVSQHVQCYV N NGYEAEIS SYVDSDEYYE RFGEDTVPYE QFRGTYMTAE DFNRMVSMYG APGQSDKSLT SRARSTGVAN SNKVLSLEGA GR SSKTVGR VATNTASSLT SVKSGIPPRP DIDQPRGQSS KRLVGRRLEI VPGSYMYLSP AEAAEYRAQQ AAVSQVSAAF SAD VQSKMA QVSKLKR

+
Macromolecule #27: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associat...

MacromoleculeName: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod
type: protein_or_peptide / ID: 27 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 45.655566 KDa
Recombinant expressionOrganism: Porphyridium purpureum (eukaryote)
SequenceString: MRSCRRSVAQ HPCAIQVDIV AMAAFISSFA GAQLAHARPS KQTALRAALT LPGTAETNLA VPSNAVRKVQ PYPIAKPPSY SSVDSLRPA RVSRMDADWA TIYEQVRRQV MGNAYVMEGE APDIDVAFSQ LKGGNLTVRE FVRAVGKSAS YRTRFMEAKS S YNFVLLNF ...String:
MRSCRRSVAQ HPCAIQVDIV AMAAFISSFA GAQLAHARPS KQTALRAALT LPGTAETNLA VPSNAVRKVQ PYPIAKPPSY SSVDSLRPA RVSRMDADWA TIYEQVRRQV MGNAYVMEGE APDIDVAFSQ LKGGNLTVRE FVRAVGKSAS YRTRFMEAKS S YNFVLLNF KHFLGRAPTQ EEVSTHIQIL ATSGLEAEID SYIDSDEYKA LFGDHVVPYV VYRGTYLSSE RFNRMVKANP GG ATSDKAK SNLNMIATVA ADLPTDAIDV MRGLPSPITS ETLAFGTAYY WAKVEKEASE GRSASPIGEK IGKFDHAPIS TYT SLCSYD KVNKAPQISV TNVGSDEHSY VSVTSKYIAP DMAAAAQMLA DCQKYKAGGN APTGKWMKYY PGTTVNMAPY ISLN DTGSD SSRTVSVTLD KVKIS

+
Macromolecule #28: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associat...

MacromoleculeName: Phycobilisome 31.8 kDa linker polypeptide, phycoerythrin-associated, rod
type: protein_or_peptide / ID: 28 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 55.146727 KDa
Recombinant expressionOrganism: Porphyridium purpureum (eukaryote)
SequenceString: MAFVGAMPGG GGEPRAVRMM MQTDARSLSS GSSAAVPFST AVRFESPSGG LDRYSRVDPA APGPNVITRF LFKDRPVRRS DPSLSEVDR EATMRTVYRN VMGNAYVMEE ERAELATLES QFLVGAISTR DFVRGVAKSA TYKKRFFESV SQFRFIELNF K HFMGRAPL ...String:
MAFVGAMPGG GGEPRAVRMM MQTDARSLSS GSSAAVPFST AVRFESPSGG LDRYSRVDPA APGPNVITRF LFKDRPVRRS DPSLSEVDR EATMRTVYRN VMGNAYVMEE ERAELATLES QFLVGAISTR DFVRGVAKSA TYKKRFFESV SQFRFIELNF K HFMGRAPL DMAEMSKHYE IFAAGGYDAE VDSYFDSEEY LDVFGLDTVP YMRFRGTYAP NSTFNLQCRL QGGWARSDKK LP MMSMLPL NNKAAIMPHQ IVDGLPVIPN SEHPSQKYNV PKVSREKLQR ELLIAQGKAN ALQIELDAAY TSLASSRAFL APF AAMAAD MDIRPLYGKN PQVFAGQFLG VGAGQWGKTG ADTVRGRSRR VAADIGVKEF QLERVKQLVV DLQRALALED AEAD APATS LLQAYQAKVY VKPPVIAKKK GPEPVNEDEI TIGQGDKKIK VTVLRNLGDR TEKLREKPEK EEEEGPRTFK DLYET AKPM KGFPGDGSEM NVGG

+
Macromolecule #29: FAS1 domain-containing protein

MacromoleculeName: FAS1 domain-containing protein / type: protein_or_peptide / ID: 29 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 31.332955 KDa
Recombinant expressionOrganism: Porphyridium purpureum (eukaryote)
SequenceString: MAAKPYESGY IAEDDFWRGR GIAAWVYATG ANKVIAQIVK DFNLTDKKFM VFIPNDGAFA RLSPQLRKAM MEDSRLVYDM LAGHIFTSK GSAMLKDLQG AGYLQPAYGE AIGYVGTGRV IKIGNAQVIP ESSDILRKNL GFSAHTLDTF IVPKALTKKV S IEAGFSPV ...String:
MAAKPYESGY IAEDDFWRGR GIAAWVYATG ANKVIAQIVK DFNLTDKKFM VFIPNDGAFA RLSPQLRKAM MEDSRLVYDM LAGHIFTSK GSAMLKDLQG AGYLQPAYGE AIGYVGTGRV IKIGNAQVIP ESSDILRKNL GFSAHTLDTF IVPKALTKKV S IEAGFSPV TPAKYVSTTK ADLRYVGATK PAAVGGRRAM NLMKQQPFWM YGPPYNAVTQ DEYEPISAAA PKAFVDYQIF AP GTVKVSP DSVNANELNP VSGMSKYIGK TQKLVGDQGI SDRSDKLPMG Q

+
Macromolecule #30: LPP2

MacromoleculeName: LPP2 / type: protein_or_peptide / ID: 30 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Porphyridium purpureum (eukaryote)
Molecular weightTheoretical: 17.273779 KDa
Recombinant expressionOrganism: Porphyridium purpureum (eukaryote)
SequenceString:
MVVVCASRKS GRAVVPAMVP ALRVELVVSK QMVMGLAALF FQVQRTALMA KLELPKFSMP SMPSMPKLTV PKLQMPKLGG GEKKDKAAK PSPSAPKTTI RPSGGVKVRA AVGNKSNVDA PSFKGSNMEL ADSGADYKAF PKRRMPGANM QGFLDMAKGM K PK

+
Macromolecule #31: PHYCOERYTHROBILIN

MacromoleculeName: PHYCOERYTHROBILIN / type: ligand / ID: 31 / Number of copies: 1434 / Formula: PEB
Molecular weightTheoretical: 588.694 Da
Chemical component information

ChemComp-PEB:
PHYCOERYTHROBILIN / Phycoerythrobilin

+
Macromolecule #32: PHYCOUROBILIN

MacromoleculeName: PHYCOUROBILIN / type: ligand / ID: 32 / Number of copies: 48 / Formula: PUB
Molecular weightTheoretical: 590.71 Da
Chemical component information

ChemComp-CYB:
PHYCOUROBILIN / Phycourobilin

+
Macromolecule #33: PHYCOCYANOBILIN

MacromoleculeName: PHYCOCYANOBILIN / type: ligand / ID: 33 / Number of copies: 120 / Formula: CYC
Molecular weightTheoretical: 588.694 Da
Chemical component information

ChemComp-CYC:
PHYCOCYANOBILIN / Phycocyanobilin

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Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation statecell

-
Sample preparation

BufferpH: 7
VitrificationCryogen name: ETHANE

-
Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 6.0 µm / Nominal defocus min: 1.0 µm
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 35.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

-
Image processing

Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

6kgx

Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.3 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 215000

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