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Structure paper

TitleIn situ structure of the red algal phycobilisome-PSII-PSI-LHC megacomplex.
Journal, issue, pagesNature, Vol. 616, Issue 7955, Page 199-206, Year 2023
Publish dateMar 15, 2023
AuthorsXin You / Xing Zhang / Jing Cheng / Yanan Xiao / Jianfei Ma / Shan Sun / Xinzheng Zhang / Hong-Wei Wang / Sen-Fang Sui /
PubMed AbstractIn oxygenic photosynthetic organisms, light energy is captured by antenna systems and transferred to photosystem II (PSII) and photosystem I (PSI) to drive photosynthesis. The antenna systems of red ...In oxygenic photosynthetic organisms, light energy is captured by antenna systems and transferred to photosystem II (PSII) and photosystem I (PSI) to drive photosynthesis. The antenna systems of red algae consist of soluble phycobilisomes (PBSs) and transmembrane light-harvesting complexes (LHCs). Excitation energy transfer pathways from PBS to photosystems remain unclear owing to the lack of structural information. Here we present in situ structures of PBS-PSII-PSI-LHC megacomplexes from the red alga Porphyridium purpureum at near-atomic resolution using cryogenic electron tomography and in situ single-particle analysis, providing interaction details between PBS, PSII and PSI. The structures reveal several unidentified and incomplete proteins and their roles in the assembly of the megacomplex, as well as a huge and sophisticated pigment network. This work provides a solid structural basis for unravelling the mechanisms of PBS-PSII-PSI-LHC megacomplex assembly, efficient energy transfer from PBS to the two photosystems, and regulation of energy distribution between PSII and PSI.
External linksNature / PubMed:36922595
MethodsEM (single particle)
Resolution3.3 - 6.3 Å
Structure data

33558

7y1a

EMDB-33558, PDB-7y1a:
Lateral hexamer
Method: EM (single particle) / Resolution: 6.3 Å

33605

7y4l

EMDB-33605, PDB-7y4l:
PBS of PBS-PSII-PSI-LHCs from Porphyridium purpureum.
Method: EM (single particle) / Resolution: 3.3 Å

33618

7y5e

EMDB-33618, PDB-7y5e:
In situ single-PBS-PSII-PSI-LHCs megacomplex.
Method: EM (single particle) / Resolution: 3.3 Å

33658

7y7a

EMDB-33658, PDB-7y7a:
In situ double-PBS-PSII-PSI-LHCs megacomplex from Porphyridium purpureum.
Method: EM (single particle) / Resolution: 4.3 Å

EMDB-33669: In situ double-PBS-PSII-PSI-LHCs megacomplex from red alga Porphyridium purpureum.
Method: EM (single particle) / Resolution: 4.3 Å

Chemicals

ChemComp-PEB:
PHYCOERYTHROBILIN / Phycoerythrobilin

ChemComp-PUB:
PHYCOUROBILIN / Phycourobilin

ChemComp-CYC:
PHYCOCYANOBILIN / Phycocyanobilin

ChemComp-BCR:
BETA-CAROTENE / Β-Carotene

ChemComp-CL:
Unknown entry / Chloride

ChemComp-CLA:
CHLOROPHYLL A / Chlorophyll a

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM / Phosphatidylglycerol

ChemComp-OEX:
CA-MN4-O5 CLUSTER

ChemComp-FE:
Unknown entry / Iron

ChemComp-PHO:
PHEOPHYTIN A / Pheophytin

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

ChemComp-BCT:
BICARBONATE ION / pH buffer*YM / Bicarbonate

ChemComp-PL9:
2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE / Plastoquinone

ChemComp-DGD:
DIGALACTOSYL DIACYL GLYCEROL (DGDG)

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE / Heme B

ChemComp-GOL:
GLYCEROL / Glycerol

ChemComp-PQN:
PHYLLOQUINONE / Phytomenadione

ChemComp-SF4:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

ChemComp-3XQ:
(2S)-2,3-dihydroxypropyl octadecanoate

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER / Iron–sulfur cluster

ChemComp-BGC:
beta-D-glucopyranose / Glucose

ChemComp-ZEX:
(1R,2S)-4-{(1E,3E,5E,7E,9E,11E,13E,15E,17E)-18-[(4S)-4-hydroxy-2,6,6-trimethylcyclohex-1-en-1-yl]-3,7,12,16-tetramethyloctadeca-1,3,5,7,9,11,13,15,17-nonaen-1-yl}-2,5,5-trimethylcyclohex-3-en-1-ol

ChemComp-HOH:
WATER / Water

Source
  • porphyridium purpureum (eukaryote)
KeywordsPHOTOSYNTHESIS / Lateral hexamer / PBS / complex / Double-PBS-PSII-PSI-LHCs

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