7F62

Cryo-EM structure of SARS-CoV-2 spike in complex with a neutralizing antibody chAb-25 (Focused refinement of S-RBD and chAb-25 region)

Summary for 7F62

• Entry DOI: 10.2210/pdb7f62/pdb
• Related: 7EJ4
• EMDB information: 30669 31470
• Descriptor: Spike glycoprotein, RBD-chAb-25, Heavy chain, RBD-chAb-25, Light chain (3 entities in total)
• Functional Keywords: sars-cov-2, spike protein, neutralizing antibody, viral protein
• Biological source: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV, SARS-CoV-2); More
• Total number of polymer chains: 3
• Total formula weight: 214605.14

Authors

Yang, T.J.,Yu, P.Y.,Wu, H.C.,Hsu, S.T.D. (deposition date: 2021-06-24, release date: 2021-08-04, Last modification date: 2024-10-16)

Primary citation

Su, S.C.,Yang, T.J.,Yu, P.Y.,Liang, K.H.,Chen, W.Y.,Yang, C.W.,Lin, H.T.,Wang, M.J.,Lu, R.M.,Tso, H.C.,Chung, M.J.,Hsieh, T.Y.,Chang, Y.L.,Lin, S.C.,Hsu, F.Y.,Ke, F.Y.,Wu, Y.H.,Hwang, Y.C.,Liu, I.J.,Liang, J.J.,Liao, C.C.,Ko, H.Y.,Sun, C.P.,Wu, P.Y.,Jan, J.T.,Chang, Y.C.,Lin, Y.L.,Tao, M.H.,Hsu, S.D.,Wu, H.C.
Structure-guided antibody cocktail for prevention and treatment of COVID-19.
Plos Pathog., 17:e1009704-e1009704, 2021

PubMed Abstract: Development of effective therapeutics for mitigating the COVID-19 pandemic is a pressing global need. Neutralizing antibodies are known to be effective antivirals, as they can be rapidly deployed to prevent disease progression and can accelerate patient recovery without the need for fully developed host immunity. Here, we report the generation and characterization of a series of chimeric antibodies against the receptor-binding domain (RBD) of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein. Some of these antibodies exhibit exceptionally potent neutralization activities in vitro and in vivo, and the most potent of our antibodies target three distinct non-overlapping epitopes within the RBD. Cryo-electron microscopy analyses of two highly potent antibodies in complex with the SARS-CoV-2 spike protein suggested they may be particularly useful when combined in a cocktail therapy. The efficacy of this antibody cocktail was confirmed in SARS-CoV-2-infected mouse and hamster models as prophylactic and post-infection treatments. With the emergence of more contagious variants of SARS-CoV-2, cocktail antibody therapies hold great promise to control disease and prevent drug resistance.

PubMed: 34673836
DOI: 10.1371/journal.ppat.1009704

Experimental method

ELECTRON MICROSCOPY (3.6 Å)