7BFZ

X-ray structure of human prostate-specific membrane antigen(PSMA) in complex with a inhibitor Glu-490

Summary for 7BFZ

• Entry DOI: 10.2210/pdb7bfz/pdb
• Descriptor: Glutamate carboxypeptidase 2, 1,2-ETHANEDIOL, DI(HYDROXYETHYL)ETHER, ... (14 entities in total)
• Functional Keywords: glutamate carboxypeptidase ii (gcpii); naaladase; prostate-specific membrane antigen (psma); inhibitor, hydrolase
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 1
• Total formula weight: 84540.56

Authors

Rakhimbekova, A.,Motlova, L.,Barinka, C. (deposition date: 2021-01-05, release date: 2021-08-18, Last modification date: 2024-11-06)

Primary citation

Zhang, J.,Rakhimbekova, A.,Duan, X.,Yin, Q.,Foss, C.A.,Fan, Y.,Xu, Y.,Li, X.,Cai, X.,Kutil, Z.,Wang, P.,Yang, Z.,Zhang, N.,Pomper, M.G.,Wang, Y.,Barinka, C.,Yang, X.
A prostate-specific membrane antigen activated molecular rotor for real-time fluorescence imaging.
Nat Commun, 12:5460-5460, 2021

PubMed Abstract: Surgery is an efficient way to treat localized prostate cancer (PCa), however, it is challenging to demarcate rapidly and accurately the tumor boundary intraoperatively, as existing tumor detection methods are seldom performed in real-time. To overcome those limitations, we develop a fluorescent molecular rotor that specifically targets the prostate-specific membrane antigen (PSMA), an established marker for PCa. The probes have picomolar affinity (IC= 63-118 pM) for PSMA and generate virtually instantaneous onset of robust fluorescent signal proportional to the concentration of the PSMA-probe complex. In vitro and ex vivo experiments using PCa cell lines and clinical samples, respectively, indicate the utility of the probe for biomedical applications, including real-time monitoring of endocytosis and tumor staging. Experiments performed in a PCa xenograft model reveal suitability of the probe for imaging applications in vivo.

PubMed: 34526506
DOI: 10.1038/s41467-021-25746-6

Experimental method

X-RAY DIFFRACTION (1.73 Å)