6ZZ6

Cryo-EM structure of S.cerevisiae cohesin-Scc2-DNA complex

6ZZ6 の概要

• エントリーDOI: 10.2210/pdb6zz6/pdb
• EMDBエントリー: 11585
• 分子名称: Structural maintenance of chromosomes protein 1,Structural maintenance of chromosomes protein 1,Structural maintenance of chromosomes protein 1, Structural maintenance of chromosomes protein 3,Structural maintenance of chromosomes protein 3,Structural maintenance of chromosomes protein 3, Sister chromatid cohesion protein 1,Sister chromatid cohesion protein 1,Sister chromatid cohesion protein 1, ... (8 entities in total)
• 機能のキーワード: smc, cohesin, dna, cell cycle
• 由来する生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 291548.38

構造登録者

Lee, B.-G.,Gonzalez Llamazares, A.,Collier, J.,Nasmyth, K.A.,Lowe, J. (登録日: 2020-08-04, 公開日: 2020-09-30, 最終更新日: 2024-05-01)

主引用文献

Collier, J.E.,Lee, B.G.,Roig, M.B.,Yatskevich, S.,Petela, N.J.,Metson, J.,Voulgaris, M.,Gonzalez Llamazares, A.,Lowe, J.,Nasmyth, K.A.
Transport of DNA within cohesin involves clamping on top of engaged heads by Scc2 and entrapment within the ring by Scc3.
Elife, 9:-, 2020

PubMed Abstract: In addition to extruding DNA loops, cohesin entraps within its SMC-kleisin ring (S-K) individual DNAs during G1 and sister DNAs during S-phase. All three activities require related hook-shaped proteins called Scc2 and Scc3. Using thiol-specific crosslinking we provide rigorous proof of entrapment activity in vitro. Scc2 alone promotes entrapment of DNAs in the E-S and E-K compartments, between ATP-bound engaged heads and the SMC hinge and associated kleisin, respectively. This does not require ATP hydrolysis nor is it accompanied by entrapment within S-K rings, which is a slower process requiring Scc3. Cryo-EM reveals that DNAs transported into E-S/E-K compartments are 'clamped' in a sub-compartment created by Scc2's association with engaged heads whose coiled coils are folded around their elbow. We suggest that clamping may be a recurrent feature of cohesin complexes active in loop extrusion and that this conformation precedes the S-K entrapment required for sister chromatid cohesion.

PubMed: 32930661
DOI: 10.7554/eLife.59560

実験手法

ELECTRON MICROSCOPY (3.4 Å)