6ZV6

Human RIO1(kd)-StHA late pre-40S particle, structural state B (post 18S rRNA cleavage)

6ZV6 の概要

• エントリーDOI: 10.2210/pdb6zv6/pdb
• 関連するPDBエントリー: 6ZUO
• EMDBエントリー: 11440 11441 11442 11443 11444 11445 11446 11447
• 分子名称: 18S ribosomal RNA, 40S ribosomal protein S8, 40S ribosomal protein S9, ... (39 entities in total)
• 機能のキーワード: human ribosome biogenesis, small ribosomal subunit, rrna processing, ribosome
• 由来する生物種: Homo sapiens; 詳細
• タンパク質・核酸の鎖数: 35
• 化学式量合計: 1288351.50

構造登録者

Plassart, L.,Shayan, R.,Plisson-Chastang, C. (登録日: 2020-07-24, 公開日: 2021-05-12, 最終更新日: 2025-04-09)

主引用文献

Plassart, L.,Shayan, R.,Montellese, C.,Rinaldi, D.,Larburu, N.,Pichereaux, C.,Froment, C.,Lebaron, S.,O'Donohue, M.F.,Kutay, U.,Marcoux, J.,Gleizes, P.E.,Plisson-Chastang, C.
The final step of 40S ribosomal subunit maturation is controlled by a dual key lock.
Elife, 10:-, 2021

PubMed Abstract: Preventing premature interaction of pre-ribosomes with the translation apparatus is essential for translational accuracy. Hence, the final maturation step releasing functional 40S ribosomal subunits, namely processing of the 18S ribosomal RNA 3' end, is safeguarded by the protein DIM2, which both interacts with the endoribonuclease NOB1 and masks the rRNA cleavage site. To elucidate the control mechanism that unlocks NOB1 activity, we performed cryo-electron microscopy analysis of late human pre-40S particles purified using a catalytically inactive form of the ATPase RIO1. These structures, together with in vivo and in vitro functional analyses, support a model in which ATP-loaded RIO1 cooperates with ribosomal protein RPS26/eS26 to displace DIM2 from the 18S rRNA 3' end, thereby triggering final cleavage by NOB1; release of ADP then leads to RIO1 dissociation from the 40S subunit. This dual key lock mechanism requiring RIO1 and RPS26 guarantees the precise timing of pre-40S particle conversion into translation-competent ribosomal subunits.

PubMed: 33908345
DOI: 10.7554/eLife.61254

実験手法

ELECTRON MICROSCOPY (2.9 Å)