6ZUI

Crystal structure of the Cys-Ser mutant of the cpYFP-based biosensor for hypochlorous acid

6ZUI の概要

• エントリーDOI: 10.2210/pdb6zui/pdb
• 分子名称: HTH-type transcriptional repressor NemR,Green fluorescent protein,Green fluorescent protein,HTH-type transcriptional repressor NemR (2 entities in total)
• 機能のキーワード: biosensor, hypohalous acid, cpyfp, yellow fluorescent protein, fluorescent protein
• 由来する生物種: Escherichia coli (strain K12); 詳細
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 49632.64

構造登録者

Tossounian, M.A.,Van Molle, I.,Messens, J. (登録日: 2020-07-23, 公開日: 2021-06-30, 最終更新日: 2024-10-16)

主引用文献

Kostyuk, A.I.,Tossounian, M.A.,Panova, A.S.,Thauvin, M.,Raevskii, R.I.,Ezerina, D.,Wahni, K.,Van Molle, I.,Sergeeva, A.D.,Vertommen, D.,Gorokhovatsky, A.Y.,Baranov, M.S.,Vriz, S.,Messens, J.,Bilan, D.S.,Belousov, V.V.
Hypocrates is a genetically encoded fluorescent biosensor for (pseudo)hypohalous acids and their derivatives.
Nat Commun, 13:171-171, 2022

PubMed Abstract: The lack of tools to monitor the dynamics of (pseudo)hypohalous acids in live cells and tissues hinders a better understanding of inflammatory processes. Here we present a fluorescent genetically encoded biosensor, Hypocrates, for the visualization of (pseudo)hypohalous acids and their derivatives. Hypocrates consists of a circularly permuted yellow fluorescent protein integrated into the structure of the transcription repressor NemR from Escherichia coli. We show that Hypocrates is ratiometric, reversible, and responds to its analytes in the 10 Ms range. Solving the Hypocrates X-ray structure provided insights into its sensing mechanism, allowing determination of the spatial organization in this circularly permuted fluorescent protein-based redox probe. We exemplify its applicability by imaging hypohalous stress in bacteria phagocytosed by primary neutrophils. Finally, we demonstrate that Hypocrates can be utilized in combination with HyPerRed for the simultaneous visualization of (pseudo)hypohalous acids and hydrogen peroxide dynamics in a zebrafish tail fin injury model.

PubMed: 35013284
DOI: 10.1038/s41467-021-27796-2

実験手法

X-RAY DIFFRACTION (2.20008218184 Å)