6ZOZ

Structure of Disulphide-stabilized SARS-CoV-2 Spike Protein Trimer (x1 disulphide-bond mutant, S383C, D985C, K986P, V987P, single Arg S1/S2 cleavage site) in Locked State

6ZOZ の概要

• エントリーDOI: 10.2210/pdb6zoz/pdb
• EMDBエントリー: 11331
• 分子名称: Spike glycoprotein, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total)
• 機能のキーワード: coronavirus, sars-cov-2, spike protein, s protein, s antigen, covid-19, receptor binding, membrane fusion, vaccine design, viral protein
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 430316.68

構造登録者

Xiong, X.,Qu, K.,Scheres, S.H.W.,Briggs, J.A.G. (登録日: 2020-07-08, 公開日: 2020-07-22, 最終更新日: 2024-10-16)

主引用文献

Xiong, X.,Qu, K.,Ciazynska, K.A.,Hosmillo, M.,Carter, A.P.,Ebrahimi, S.,Ke, Z.,Scheres, S.H.W.,Bergamaschi, L.,Grice, G.L.,Zhang, Y.,Nathan, J.A.,Baker, S.,James, L.C.,Baxendale, H.E.,Goodfellow, I.,Doffinger, R.,Briggs, J.A.G.
A thermostable, closed SARS-CoV-2 spike protein trimer.
Nat.Struct.Mol.Biol., 27:934-941, 2020

PubMed Abstract: The spike (S) protein of SARS-CoV-2 mediates receptor binding and cell entry and is the dominant target of the immune system. It exhibits substantial conformational flexibility. It transitions from closed to open conformations to expose its receptor-binding site and, subsequently, from prefusion to postfusion conformations to mediate fusion of viral and cellular membranes. S-protein derivatives are components of vaccine candidates and diagnostic assays, as well as tools for research into the biology and immunology of SARS-CoV-2. Here we have designed mutations in S that allow the production of thermostable, disulfide-bonded S-protein trimers that are trapped in the closed, prefusion state. Structures of the disulfide-stabilized and non-disulfide-stabilized proteins reveal distinct closed and locked conformations of the S trimer. We demonstrate that the designed, thermostable, closed S trimer can be used in serological assays. This protein has potential applications as a reagent for serology, virology and as an immunogen.

PubMed: 32737467
DOI: 10.1038/s41594-020-0478-5

実験手法

ELECTRON MICROSCOPY (3.5 Å)