6ZNI

Structure of the Shigella MxiH needle filament attached to the basal body

Summary for 6ZNI

• Entry DOI: 10.2210/pdb6zni/pdb
• EMDB information: 11312
• Descriptor: Protein MxiH (1 entity in total)
• Functional Keywords: type 3 secretion system, shigella, helical reconstruction, needle filament., protein transport
• Biological source: Shigella flexneri 5a str. M90T
• Total number of polymer chains: 23
• Total formula weight: 254386.42

Authors

Lunelli, M.,Kotov, V.,Marlovits, T.C.,Kolbe, M. (deposition date: 2020-07-06, release date: 2021-07-07, Last modification date: 2024-05-01)

Primary citation

Kotov, V.,Lunelli, M.,Wald, J.,Kolbe, M.,Marlovits, T.C.
Helical reconstruction of Salmonella and Shigella needle filaments attached to type 3 basal bodies.
Biochem Biophys Rep, 27:101039-101039, 2021

PubMed Abstract: Gram-negative pathogens evolved a syringe-like nanomachine, termed type 3 secretion system, to deliver protein effectors into the cytoplasm of host cells. An essential component of this system is a long helical needle filament that protrudes from the bacterial surface and connects the cytoplasms of the bacterium and the eukaryotic cell. Previous structural research was predominantly focused on reconstituted type 3 needle filaments, which lacked the biological context. In this work we introduce a facile procedure to obtain high-resolution cryo-EM structure of needle filaments attached to the basal body of type 3 secretion systems. We validate our approach by solving the structure of PrgI filament and demonstrate its utility by obtaining the first high-resolution cryo-EM reconstruction of Shigella MxiH filament. Our work paves the way to systematic structural characterization of attached type 3 needle filaments in the context of mutagenesis studies, protein structural evolution and drug development.

PubMed: 34258394
DOI: 10.1016/j.bbrep.2021.101039

Experimental method

ELECTRON MICROSCOPY (3.6 Å)