6ZLF

Aerobic crystal structure of F420H2-Oxidase from Methanothermococcus thermolithotrophicus at 1.8A resolution under 125 bars of krypton

Summary for 6ZLF

Entry DOI	10.2210/pdb6zlf/pdb
Related	6ZK8
Descriptor	Coenzyme F420H2 oxidase (FprA), HEXANE-1,6-DIOL, MU-OXO-DIIRON, ... (7 entities in total)
Functional Keywords	krypton derivatization, oxidase, o2, oxidoreductase
Biological source	Methanothermococcus thermolithotrophicus
Total number of polymer chains	8
Total formula weight	381515.86
Authors	Engilberge, S.,Wagner, T.,Carpentier, P.,Girard, E.,Shima, S. (deposition date: 2020-06-30, release date: 2020-11-25, Last modification date: 2024-01-31)
Primary citation	Engilberge, S.,Wagner, T.,Carpentier, P.,Girard, E.,Shima, S. Krypton-derivatization highlights O 2 -channeling in a four-electron reducing oxidase. Chem.Commun.(Camb.), 56:10863-10866, 2020 Cited by PubMed Abstract: F420H2-oxidase (FprA) catalyses the four-electron reduction of O2 to 2H2O using the reduced form of F420 as electron donor. The hydrophobic O2-channel detected by Kr-derivatization and the concerted movement of a gating loop could contribute to prevent unwanted side-reaction between the catalytic intermediates and solvents, therefore preventing reactive oxygen species formation. PubMed: 32940290 DOI: 10.1039/d0cc04557h PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.8 Å)

Structure validation