6ZE9

Non-native fold of the putative VPS39 zinc finger domain

Summary for 6ZE9

• Entry DOI: 10.2210/pdb6ze9/pdb
• Descriptor: Vam6/Vps39-like protein, ZINC ION (3 entities in total)
• Functional Keywords: membrane trafficking, hops, endocytosis
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 3
• Total formula weight: 15418.06

Authors

Butt, B.G.,Graham, S.C. (deposition date: 2020-06-16, release date: 2020-06-24, Last modification date: 2024-10-23)

Primary citation

Butt, B.G.,Scourfield, E.J.,Graham, S.C.
Non-native fold of the putative VPS39 zinc finger domain.
Wellcome Open Res, 5:154-154, 2020

PubMed Abstract: The multi-subunit homotypic fusion and vacuole protein sorting (HOPS) membrane-tethering complex is involved in regulating the fusion of late endosomes and autophagosomes with lysosomes in eukaryotes. The C-terminal regions of several HOPS components have been shown to be required for correct complex assembly, including the C-terminal really interesting new gene (RING) zinc finger domains of HOPS components VPS18 and VPS41. We sought to structurally characterise the putative C-terminal zinc finger domain of VPS39, which we hypothesised may be important for binding of VPS39 to cellular partners or to other HOPS components. We recombinantly expressed, purified and solved the crystal structure of the proposed zinc-binding region of VPS39. In the structure, this region forms an anti-parallel β-hairpin that is incorporated into a homotetrameric eight-stranded β-barrel. However, the fold is stabilised by coordination of zinc ions by residues from the purification tag and an intramolecular disulphide bond between two predicted zinc ligands. We solved the structure of the VPS39 C-terminal domain adopting a non-native fold. Our work highlights the risk of non-native folds when purifying small zinc-containing domains with hexahistidine tags. However, the non-native structure we observe may have implications for rational protein design.

PubMed: 32724865
DOI: 10.12688/wellcomeopenres.16078.1

Experimental method

X-RAY DIFFRACTION (2.9 Å)