6ZDG
Association of three complexes of largely structurally disordered Spike ectodomain with bound EY6A Fab
Summary for 6ZDG
| Entry DOI | 10.2210/pdb6zdg/pdb |
| Related | 6ZCZ |
| EMDB information | 11173 |
| Descriptor | Spike protein S1, EY6A heavy chain, EY6A light chain, ... (4 entities in total) |
| Functional Keywords | ey6a, rbd, spike glycoprotein, sars-cov-2, human neutralizing antibody, viral protein |
| Biological source | Severe acute respiratory syndrome coronavirus 2 More |
| Total number of polymer chains | 9 |
| Total formula weight | 208979.15 |
| Authors | Duyvesteyn, H.M.E.,Zhou, D.,Zhao, Y.,Fry, E.E.,Ren, J.,Stuart, D.I. (deposition date: 2020-06-14, release date: 2020-07-29, Last modification date: 2024-11-20) |
| Primary citation | Zhou, D.,Duyvesteyn, H.M.E.,Chen, C.P.,Huang, C.G.,Chen, T.H.,Shih, S.R.,Lin, Y.C.,Cheng, C.Y.,Cheng, S.H.,Huang, Y.C.,Lin, T.Y.,Ma, C.,Huo, J.,Carrique, L.,Malinauskas, T.,Ruza, R.R.,Shah, P.N.M.,Tan, T.K.,Rijal, P.,Donat, R.F.,Godwin, K.,Buttigieg, K.R.,Tree, J.A.,Radecke, J.,Paterson, N.G.,Supasa, P.,Mongkolsapaya, J.,Screaton, G.R.,Carroll, M.W.,Gilbert-Jaramillo, J.,Knight, M.L.,James, W.,Owens, R.J.,Naismith, J.H.,Townsend, A.R.,Fry, E.E.,Zhao, Y.,Ren, J.,Stuart, D.I.,Huang, K.A. Structural basis for the neutralization of SARS-CoV-2 by an antibody from a convalescent patient. Nat.Struct.Mol.Biol., 27:950-958, 2020 Cited by PubMed Abstract: The COVID-19 pandemic has had an unprecedented health and economic impact and there are currently no approved therapies. We have isolated an antibody, EY6A, from an individual convalescing from COVID-19 and have shown that it neutralizes SARS-CoV-2 and cross-reacts with SARS-CoV-1. EY6A Fab binds the receptor binding domain (RBD) of the viral spike glycoprotein tightly (K of 2 nM), and a 2.6-Å-resolution crystal structure of an RBD-EY6A Fab complex identifies the highly conserved epitope, away from the ACE2 receptor binding site. Residues within this footprint are key to stabilizing the pre-fusion spike. Cryo-EM analyses of the pre-fusion spike incubated with EY6A Fab reveal a complex of the intact spike trimer with three Fabs bound and two further multimeric forms comprising the destabilized spike attached to Fab. EY6A binds what is probably a major neutralizing epitope, making it a candidate therapeutic for COVID-19. PubMed: 32737466DOI: 10.1038/s41594-020-0480-y PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (4.7 Å) |
Structure validation
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