6ZBM

Structure of the D125N mutant of the catalytic domain of the Bacillus circulans alpha-1,6 Mannanase in complex with an alpha-1,6-alpha-manno-cyclophellitol carbasugar-stabilised trisaccharide inhibitor

Summary for 6ZBM

Entry DOI	10.2210/pdb6zbm/pdb
Descriptor	Alpha-1,6-mannanase, 1,2-ETHANEDIOL, (1~{R},2~{R},3~{R},4~{S},5~{R})-4-[[(1~{S},2~{S},3~{S},4~{R},5~{R})-5-(hydroxymethyl)-2,3,4-tris(oxidanyl)cyclohexyl]oxymethyl]cyclohexane-1,2,3,5-tetrol, ... (5 entities in total)
Functional Keywords	endo-alpha-1, 6-mannanase, epoxide, inhibitor, hydrolase
Biological source	Bacillus circulans
Total number of polymer chains	1
Total formula weight	41572.52
Authors	Davies, G.J.,Offen, W.A. (deposition date: 2020-06-08, release date: 2021-04-28, Last modification date: 2024-01-24)
Primary citation	Schroder, S.P.,Offen, W.A.,Males, A.,Jin, Y.,de Boer, C.,Enotarpi, J.,Marino, L.,van der Marel, G.A.,Florea, B.I.,Codee, J.D.C.,Overkleeft, H.S.,Davies, G.J. Development of Non-Hydrolysable Oligosaccharide Activity-Based Inactivators for Endoglycanases: A Case Study on alpha-1,6 Mannanases. Chemistry, 27:9519-9523, 2021 Cited by PubMed: 33878235 DOI: 10.1002/chem.202101255 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.47 Å)

Structure validation