6Z6W
Poliovirus type 3 (strain Saukett) stabilised virus-like particle (PV3 SC8) from a mammalian expression system.
Summary for 6Z6W
| Entry DOI | 10.2210/pdb6z6w/pdb |
| EMDB information | 11106 |
| Descriptor | Capsid proteins, VP1, Capsid proteins, VP0, Capsid proteins, VP3, ... (4 entities in total) |
| Functional Keywords | capsid protein, virus like particle |
| Biological source | Human poliovirus 3 More |
| Total number of polymer chains | 3 |
| Total formula weight | 97800.40 |
| Authors | Bahar, M.W.,Porta, C.,Fry, E.E.,Stuart, D.I. (deposition date: 2020-05-29, release date: 2020-12-30, Last modification date: 2024-07-10) |
| Primary citation | Bahar, M.W.,Porta, C.,Fox, H.,Macadam, A.J.,Fry, E.E.,Stuart, D.I. Mammalian expression of virus-like particles as a proof of principle for next generation polio vaccines. NPJ Vaccines, 6:5-5, 2021 Cited by PubMed Abstract: Global vaccination programs using live-attenuated oral and inactivated polio vaccine (OPV and IPV) have almost eradicated poliovirus (PV) but these vaccines or their production pose significant risk in a polio-free world. Recombinant PV virus-like particles (VLPs), lacking the viral genome, represent safe next-generation vaccines, however their production requires optimisation. Here we present an efficient mammalian expression strategy producing good yields of wild-type PV VLPs for all three serotypes and a thermostabilised variant for PV3. Whilst the wild-type VLPs were predominantly in the non-native C-antigenic form, the thermostabilised PV3 VLPs adopted the native D-antigenic conformation eliciting neutralising antibody titres equivalent to the current IPV and were indistinguishable from natural empty particles by cryo-electron microscopy with a similar stabilising lipidic pocket-factor in the VP1 β-barrel. This factor may not be available in alternative expression systems, which may require synthetic pocket-binding factors. VLPs equivalent to these mammalian expressed thermostabilized particles, represent safer non-infectious vaccine candidates for the post-eradication era. PubMed: 33420068DOI: 10.1038/s41541-020-00267-3 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3 Å) |
Structure validation
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