6Z5H

Crystal structure of Aeromonas exotoxin A

This is a non-PDB format compatible entry.

Summary for 6Z5H

• Entry DOI: 10.2210/pdb6z5h/pdb
• Descriptor: Exotoxin, SODIUM ION, DI(HYDROXYETHYL)ETHER, ... (5 entities in total)
• Functional Keywords: adp-ribosyltransferase, bacterial toxin, exotoxin, aeromonas, toxin
• Biological source: Aeromonas hydrophila
• Total number of polymer chains: 2
• Total formula weight: 136710.80

Authors

Masuyer, G. (deposition date: 2020-05-26, release date: 2020-06-24, Last modification date: 2024-11-20)

Primary citation

Masuyer, G.
Crystal Structure of Exotoxin A from Aeromonas Pathogenic Species.
Toxins, 12:-, 2020

PubMed Abstract: exotoxin A (AE) is a bacterial virulence factor recently discovered in a clinical case of necrotising fasciitis caused by the flesh-eating . Here, database mining shows that AE is present in the genome of several emerging pathogenic species. The X-ray crystal structure of AE was solved at 2.3 Å and presents all the hallmarks common to diphthamide-specific mono-ADP-ribosylating toxins, suggesting AE is a fourth member of this family alongside the diphtheria toxin, exotoxin A and cholix. Structural homology indicates AE may use a similar mechanism of cytotoxicity that targets eukaryotic elongation factor 2 and thus inhibition of protein synthesis. The structure of AE also highlights unique features including a metal binding site, and a negatively charged cleft that could play a role in interdomain interactions and may affect toxicity. This study raises new opportunities to engineer alternative toxin-based molecules with pharmaceutical potential.

PubMed: 32549399
DOI: 10.3390/toxins12060397

Experimental method

X-RAY DIFFRACTION (2.3 Å)