6YWS

The structure of the large subunit of the mitoribosome from Neurospora crassa

これはPDB形式変換不可エントリーです。

6YWS の概要

• エントリーDOI: 10.2210/pdb6yws/pdb
• EMDBエントリー: 10973
• 分子名称: RNA (2820-MER), Ribosomal protein L13, 50S ribosomal protein L14, ... (50 entities in total)
• 機能のキーワード: neurospora crassa, translating mitoribosomes, trna, mrna, ml108, translation
• 由来する生物種: Neurospora crassa OR74A; 詳細
• タンパク質・核酸の鎖数: 45
• 化学式量合計: 2367412.41

構造登録者

Amunts, A.,Itoh, Y.,Naschberger, A. (登録日: 2020-04-30, 公開日: 2020-11-11, 最終更新日: 2024-05-22)

主引用文献

Itoh, Y.,Naschberger, A.,Mortezaei, N.,Herrmann, J.M.,Amunts, A.
Analysis of translating mitoribosome reveals functional characteristics of translation in mitochondria of fungi.
Nat Commun, 11:5187-5187, 2020

PubMed Abstract: Mitoribosomes are specialized protein synthesis machineries in mitochondria. However, how mRNA binds to its dedicated channel, and tRNA moves as the mitoribosomal subunit rotate with respect to each other is not understood. We report models of the translating fungal mitoribosome with mRNA, tRNA and nascent polypeptide, as well as an assembly intermediate. Nicotinamide adenine dinucleotide (NAD) is found in the central protuberance of the large subunit, and the ATPase inhibitory factor 1 (IF) in the small subunit. The models of the active mitoribosome explain how mRNA binds through a dedicated protein platform on the small subunit, tRNA is translocated with the help of the protein mL108, bridging it with L1 stalk on the large subunit, and nascent polypeptide paths through a newly shaped exit tunnel involving a series of structural rearrangements. An assembly intermediate is modeled with the maturation factor Atp25, providing insight into the biogenesis of the mitoribosomal large subunit and translation regulation.

PubMed: 33056988
DOI: 10.1038/s41467-020-18830-w

実験手法

ELECTRON MICROSCOPY (2.74 Å)