6YW8
NMR solution structure of unbound recombinant human Nerve Growth Factor (rhNGF)
Summary for 6YW8
| Entry DOI | 10.2210/pdb6yw8/pdb |
| NMR Information | BMRB: 34515 |
| Descriptor | Beta-nerve growth factor (1 entity in total) |
| Functional Keywords | human ngf, unbound structure, homodimer, cystin-knot, hormone |
| Biological source | Homo sapiens (Human) |
| Total number of polymer chains | 2 |
| Total formula weight | 26574.28 |
| Authors | Golic Grdadolnik, S.,Paoletti, F. (deposition date: 2020-04-29, release date: 2021-05-12, Last modification date: 2024-11-13) |
| Primary citation | Paoletti, F.,Merzel, F.,Cassetta, A.,Ogris, I.,Covaceuszach, S.,Grdadolnik, J.,Lamba, D.,Golic Grdadolnik, S. Endogenous modulators of neurotrophin signaling: Landscape of the transient ATP-NGF interactions. Comput Struct Biotechnol J, 19:2938-2949, 2021 Cited by PubMed Abstract: The Nerve Growth Factor (NGF) neurotrophin acts in the maintenance and growth of neuronal populations. Despite the detailed knowledge of NGF's role in neuron physiology, the structural and mechanistic determinants of NGF bioactivity modulated by essential endogenous ligands are still lacking. We present the results of an integrated structural and advanced computational approach to characterize the extracellular ATP-NGF interaction. We mapped by NMR the interacting surface and ATP orientation on NGF and revealed the functional role of this interaction in the binding to TrkA and p75 receptors by SPR. The role of divalent ions was explored in conjunction with ATP. Our results pinpoint ATP as a likely transient molecular modulator of NGF signaling, in health and disease states. PubMed: 34136093DOI: 10.1016/j.csbj.2021.05.009 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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