6YI3
The N-terminal RNA-binding domain of the SARS-CoV-2 nucleocapsid phosphoprotein
Summary for 6YI3
| Entry DOI | 10.2210/pdb6yi3/pdb |
| NMR Information | BMRB: 34511 |
| Descriptor | Nucleoprotein (1 entity in total) |
| Functional Keywords | covid-19, rna-binding domain, sars, mers, viral protein |
| Biological source | Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) |
| Total number of polymer chains | 1 |
| Total formula weight | 15129.85 |
| Authors | Veverka, V.,Boura, E. (deposition date: 2020-03-31, release date: 2020-04-08, Last modification date: 2024-06-19) |
| Primary citation | Dinesh, D.C.,Chalupska, D.,Silhan, J.,Koutna, E.,Nencka, R.,Veverka, V.,Boura, E. Structural basis of RNA recognition by the SARS-CoV-2 nucleocapsid phosphoprotein. Plos Pathog., 16:e1009100-e1009100, 2020 Cited by PubMed Abstract: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the coronavirus disease 2019 (COVID-19). SARS-CoV-2 is a single-stranded positive-sense RNA virus. Like other coronaviruses, SARS-CoV-2 has an unusually large genome that encodes four structural proteins and sixteen nonstructural proteins. The structural nucleocapsid phosphoprotein N is essential for linking the viral genome to the viral membrane. Both N-terminal RNA binding (N-NTD) and C-terminal dimerization domains are involved in capturing the RNA genome and, the intrinsically disordered region between these domains anchors the ribonucleoprotein complex to the viral membrane. Here, we characterized the structure of the N-NTD and its interaction with RNA using NMR spectroscopy. We observed a positively charged canyon on the surface of the N-NTD that might serve as a putative RNA binding site similarly to other coronaviruses. The subsequent NMR titrations using single-stranded and double-stranded RNA revealed a much more extensive U-shaped RNA-binding cleft lined with regularly distributed arginines and lysines. The NMR data supported by mutational analysis allowed us to construct hybrid atomic models of the N-NTD/RNA complex that provided detailed insight into RNA recognition. PubMed: 33264373DOI: 10.1371/journal.ppat.1009100 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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