6Y9W

Structure of the native full-length HIV-1 capsid protein in complex with Cyclophilin A from helical assembly (-13,8)

Summary for 6Y9W

• Entry DOI: 10.2210/pdb6y9w/pdb
• EMDB information: 10739
• Descriptor: Gag-Pol polyprotein, Peptidyl-prolyl cis-trans isomerase A (2 entities in total)
• Functional Keywords: hiv, capsid, hexamer, helical assembly, curvature, viral protein
• Biological source: Human immunodeficiency virus 1; More
• Total number of polymer chains: 13
• Total formula weight: 312278.44

Authors

Ni, T.,Gerard, S.,Zhao, G.,Ning, J.,Zhang, P. (deposition date: 2020-03-10, release date: 2020-08-19, Last modification date: 2024-11-06)

Primary citation

Ni, T.,Gerard, S.,Zhao, G.,Dent, K.,Ning, J.,Zhou, J.,Shi, J.,Anderson-Daniels, J.,Li, W.,Jang, S.,Engelman, A.N.,Aiken, C.,Zhang, P.
Intrinsic curvature of the HIV-1 CA hexamer underlies capsid topology and interaction with cyclophilin A.
Nat.Struct.Mol.Biol., 27:855-862, 2020

PubMed Abstract: The mature retrovirus capsid consists of a variably curved lattice of capsid protein (CA) hexamers and pentamers. High-resolution structures of the curved assembly, or in complex with host factors, have not been available. By devising cryo-EM methodologies for exceedingly flexible and pleomorphic assemblies, we have determined cryo-EM structures of apo-CA hexamers and in complex with cyclophilin A (CypA) at near-atomic resolutions. The CA hexamers are intrinsically curved, flexible and asymmetric, revealing the capsomere and not the previously touted dimer or trimer interfaces as the key contributor to capsid curvature. CypA recognizes specific geometries of the curved lattice, simultaneously interacting with three CA protomers from adjacent hexamers via two noncanonical interfaces, thus stabilizing the capsid. By determining multiple structures from various helical symmetries, we further revealed the essential plasticity of the CA molecule, which allows formation of continuously curved conical capsids and the mechanism of capsid pattern sensing by CypA.

PubMed: 32747784
DOI: 10.1038/s41594-020-0467-8

Experimental method

ELECTRON MICROSCOPY (4.1 Å)