6Y9P

Crystal structure of Whirlin PDZ3_C-ter in complex with Harmonin a1 C-terminal PDZ binding motif peptide

Summary for 6Y9P

• Entry DOI: 10.2210/pdb6y9p/pdb
• Descriptor: Whirlin, Harmonin a1 (3 entities in total)
• Functional Keywords: whirlin, pdz, myosin 15a, complex, structural protein
• Biological source: Mus musculus (House mouse); More
• Total number of polymer chains: 12
• Total formula weight: 76581.40

Authors

Zhu, Y.,Delhommel, F.,Haouz, A.,Caillet-Saguy, C.,Vaney, M.,Mechaly, A.E.,Wolff, N. (deposition date: 2020-03-10, release date: 2020-10-07, Last modification date: 2024-01-24)

Primary citation

Zhu, Y.,Delhommel, F.,Cordier, F.,Luchow, S.,Mechaly, A.,Colcombet-Cazenave, B.,Girault, V.,Pepermans, E.,Bahloul, A.,Gautier, C.,Brule, S.,Raynal, B.,Hoos, S.,Haouz, A.,Caillet-Saguy, C.,Ivarsson, Y.,Wolff, N.
Deciphering the Unexpected Binding Capacity of the Third PDZ Domain of Whirlin to Various Cochlear Hair Cell Partners.
J.Mol.Biol., 432:5920-5937, 2020

PubMed Abstract: Hearing is a mechanical and neurochemical process, which occurs in the hair cells of inner ear that converts the sound vibrations into electrical signals transmitted to the brain. The multi-PDZ scaffolding protein whirlin plays a critical role in the formation and function of stereocilia exposed at the surface of hair cells. In this article, we reported seven stereociliary proteins that encode PDZ binding motifs (PBM) and interact with whirlin PDZ3, where four of them are first reported. We solved the atomic resolution structures of complexes between whirlin PDZ3 and the PBMs of myosin 15a, CASK, harmonin a1 and taperin. Interestingly, the PBM of CASK and taperin are rare non-canonical PBM, which are not localized at the extreme C terminus. This large capacity to accommodate various partners could be related to the distinct functions of whirlin at different stages of the hair cell development.

PubMed: 32971111
DOI: 10.1016/j.jmb.2020.09.012

Experimental method

X-RAY DIFFRACTION (3.169 Å)