6Y8P

Crystal structure of SNAP-tag labeled with a benzyl-tetramethylrhodamine fluorophore

6Y8P の概要

• エントリーDOI: 10.2210/pdb6y8p/pdb
• 分子名称: O6-alkylguanine-DNA alkyltransferase mutant, ZINC ION, [9-[2-carboxy-5-[(4-methylphenyl)methylcarbamoyl]phenyl]-6-(dimethylamino)xanthen-3-ylidene]-dimethyl-azanium, ... (5 entities in total)
• 機能のキーワード: snap-tag, self-labeling protein, tetramethylrhodamine, synthetic fluorophore, transferase
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 19613.85

構造登録者

Gotthard, G.,Tanzer, T.,Johnsson, K.,Hiblot, J. (登録日: 2020-03-05, 公開日: 2021-03-31, 最終更新日: 2024-10-23)

主引用文献

Wilhelm, J.,Kuhn, S.,Tarnawski, M.,Gotthard, G.,Tunnermann, J.,Tanzer, T.,Karpenko, J.,Mertes, N.,Xue, L.,Uhrig, U.,Reinstein, J.,Hiblot, J.,Johnsson, K.
Kinetic and Structural Characterization of the Self-Labeling Protein Tags HaloTag7, SNAP-tag, and CLIP-tag.
Biochemistry, 60:2560-2575, 2021

PubMed Abstract: The self-labeling protein tags (SLPs) HaloTag7, SNAP-tag, and CLIP-tag allow the covalent labeling of fusion proteins with synthetic molecules for applications in bioimaging and biotechnology. To guide the selection of an SLP-substrate pair and provide guidelines for the design of substrates, we report a systematic and comparative study of the labeling kinetics and substrate specificities of HaloTag7, SNAP-tag, and CLIP-tag. HaloTag7 reaches almost diffusion-limited labeling rate constants with certain rhodamine substrates, which are more than 2 orders of magnitude higher than those of SNAP-tag for the corresponding substrates. SNAP-tag labeling rate constants, however, are less affected by the structure of the label than those of HaloTag7, which vary over 6 orders of magnitude for commonly employed substrates. Determining the crystal structures of HaloTag7 and SNAP-tag labeled with fluorescent substrates allowed us to rationalize their substrate preferences. We also demonstrate how these insights can be exploited to design substrates with improved labeling kinetics.

PubMed: 34339177
DOI: 10.1021/acs.biochem.1c00258

実験手法

X-RAY DIFFRACTION (2.3 Å)